DOI: 10.1111/j.1348-0421.1995.tb02242.x. Segmented Filamentous Bacteria Are Indigenous Intestinal Bacteria That Activate Intraepithelial Lymphocytes and Induce MHC Class II Molecules and Fucosyl Asialo GM1 Glycolipids on the Small Intestinal Epithelial Cells in the Ex‐Germ‐Free Mouse

Segmented Filamentous Bacteria Are Indigenous Intestinal Bacteria That Activate Intraepithelial Lymphocytes and Induce MHC Class II Molecules and Fucosyl Asialo GM1 Glycolipids on the Small Intestinal Epithelial Cells in the Ex‐Germ‐Free Mouse

10.1111/j.1348-0421.1995.tb02242.x

Crossref journal-article

Wiley

Microbiology and Immunology (311)

Abstract

AbstractIn ex‐germ‐free mice conventionalized by association with fecal microorganisms, the induction of major histocompatibility complex class II molecules and fucosylation of asialo GM1 glycolipid occur in the small intestinal epithelial cells (IEC). The intestinal intraepithelial lymphocytes (IEL), especially αβ T‐cell receptor‐bearing ones, also remarkably expand and show cytolytic activity. In this study, we investigated the immunological and physiological characteristics of the small intestine induced by a kind of indigenous bacteria of the small intestine, segmented filamentous bacteria (SFB), among chloroform‐resistant intestinal bacteria. Monoassociation of SFB with germ‐free mice was confirmed by the determination of the base sequences of polymerase chain reaction products of 16S rRNA genes of the fecal bacteria of these mice and in situ hybridization using fluorescein‐labeled probes based on them. SFB increased the number of αβTCR‐bearing IEL and induced Thy‐1 expression and cytolytic activity of IEL. The induction of MHC class II molecules and fucosyl asialo GM1 glycolipids and the increases in the mitotic activity and the ratio of the number of columnar cells to those of goblet cells also occurred in the small intestinal epithelial cells on monoassociation of these bacteria. SFB are important indigenous bacteria for the development of the mucosal architecture and immune system in the small intestine, at least in mice.

Bibliography

Umesaki, Y., Okada, Y., Matsumoto, S., Imaoka, A., & Setoyama, H. (1995). Segmented Filamentous Bacteria Are Indigenous Intestinal Bacteria That Activate Intraepithelial Lymphocytes and Induce MHC Class II Molecules and Fucosyl Asialo GM1 Glycolipids on the Small Intestinal Epithelial Cells in the ExâGermâFree Mouse. Microbiology and Immunology, 39(8), 555â562. Portico.

Authors 5

Yoshinori Umesaki (first)
Yasushi Okada (additional)
Satoshi Matsumoto (additional)
Akemi Imaoka (additional)
Hiromi Setoyama (additional)

References 29 Referenced 281

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{'key': 'e_1_2_2_3_1', 'first-page': '1', 'article-title': 'Antigen presentation by epithellia cells of the rat small intestine. I Kinetics, antigen specificity and blocking by anti‐Ia antisera', 'volume': '58', 'author': 'Bland P.W.', 'year': '1986', 'journal-title': 'Immunology'} / Immunology / Antigen presentation by epithellia cells of the rat small intestine. I Kinetics, antigen specificity and blocking by anti‐Ia antisera by Bland P.W. (1986)
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10.1128/iai.62.12.5442-5446.1994 / Infect. Immun. / Effects of fecal microorganisms and their chloroform‐resistants derived from mice, rats, and humans on immunological and physiological characteristics of the intestine in ex‐germ‐free mice by Okada Y. (1994)
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{'key': 'e_1_2_2_26_1', 'first-page': '441', 'article-title': 'Appl', 'volume': '47', 'author': 'Tannock G.W.', 'year': '1984', 'journal-title': 'Environ. Microbiol.'} / Environ. Microbiol. / Appl by Tannock G.W. (1984)
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{'key': 'e_1_2_2_29_1', 'first-page': '32', 'article-title': 'Expansion of αβ T‐cell receptor‐bearing intestinal intraepithelial lymphocytes after microbial colonization in germ‐free mice and its independence from thymus', 'volume': '79', 'author': 'Umesaki Y.', 'year': '1993', 'journal-title': 'Immunology'} / Immunology / Expansion of αβ T‐cell receptor‐bearing intestinal intraepithelial lymphocytes after microbial colonization in germ‐free mice and its independence from thymus by Umesaki Y. (1993)
10.1016/S0968-0004(78)80031-0

Dates

Type	When
Created	11 years, 9 months ago (Nov. 14, 2013, 12:11 p.m.)
Deposited	1 year, 9 months ago (Nov. 20, 2023, 10:08 p.m.)
Indexed	1 month ago (July 30, 2025, 10:25 a.m.)
Issued	30 years, 1 month ago (Aug. 1, 1995)
Published	30 years, 1 month ago (Aug. 1, 1995)
Published Online	11 years, 9 months ago (Nov. 14, 2013)
Published Print	30 years, 1 month ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Umesaki_1995, title={Segmented Filamentous Bacteria Are Indigenous Intestinal Bacteria That Activate Intraepithelial Lymphocytes and Induce MHC Class II Molecules and Fucosyl Asialo GM1 Glycolipids on the Small Intestinal Epithelial Cells in the Ex‐Germ‐Free Mouse}, volume={39}, ISSN={1348-0421}, url={http://dx.doi.org/10.1111/j.1348-0421.1995.tb02242.x}, DOI={10.1111/j.1348-0421.1995.tb02242.x}, number={8}, journal={Microbiology and Immunology}, publisher={Wiley}, author={Umesaki, Yoshinori and Okada, Yasushi and Matsumoto, Satoshi and Imaoka, Akemi and Setoyama, Hiromi}, year={1995}, month=aug, pages={555–562} }

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