DOI: 10.1111/j.1348-0421.1986.tb03037.x. Culture Conditions for Stimulating Cholera Toxin Production by <i>Vibrio cholerae</i> O1 El Tor

Culture Conditions for Stimulating Cholera Toxin Production by Vibrio cholerae O1 El Tor

10.1111/j.1348-0421.1986.tb03037.x

Crossref journal-article

Wiley

Microbiology and Immunology (311)

Abstract

AbstractA method that stimulates cholera toxin (CT) production by Vibrio cholerae O1 biotype El Tor (El Tor vibrios) to the level of several micrograms per ml in the culture fluid was established. Such a large amount of CT was obtained by the following method: El Tor vibrios were cultured in AKI medium (1.5% Bacto peptone, 0.4% yeast extract‐Difco, 0.5% NaCl, 0.3% NaHCO3) at 37 C for 4 hr in a stationary test tube and then for 16 hr in a shaken flask, with inoculum sizes of 105 to 107/ml. With this method, 35 strains out of 60 examined produced 2 to 16 μg/ml of CT as determined by the reversed passive latex agglutination test (RPLA). Thirty‐three randomly selected strains out of the 60 produced reasonable amounts of rabbit skin vascular permeability factor, reflecting the amount of CT titrated with RPLA.

Bibliography

Iwanaga, M., Yamamoto, K., Higa, N., Ichinose, Y., Nakasone, N., & Tanabe, M. (1986). Culture Conditions for Stimulating Cholera Toxin Production by Vibrio cholerae O1 El Tor. Microbiology and Immunology, 30(11), 1075â1083. Portico.

Authors 6

Masaaki Iwanaga (first)
Koichiro Yamamoto (additional)
Naomi Higa (additional)
Yoshio Ichinose (additional)
Noboru Nakasone (additional)
Masao Tanabe (additional)

References 15 Referenced 245

10.1038/207614a0
J.P. Craig Y. Takeda T. Miwatani 1985 KTK Scientific Publishers Tokyo
10.1111/j.1749-6632.1964.tb14213.x
10.4049/jimmunol.96.3.440 / J. Immunol. / Pathogenesis of experimental cholera: biologic activities of purified choleragen A by Finkelstein R.A. (1966)
10.1093/infdis/121.Supplement.S63
10.1128/jcm.13.1.1-5.1981 / Clin. Microbiol. / Modified Elek test for detection of heat‐labile enterotoxin of enterotoxigenic Escherichia coli. J by Honda T. (1981)
{'key': 'e_1_2_1_8_1', 'first-page': '11', 'article-title': 'Experimental study on parenteral use of antibiotics for the treatment of bacterial diarrheal diseases', 'volume': '25', 'author': 'Ichinose Y.', 'year': '1983', 'journal-title': 'Trop. Med.'} / Trop. Med. / Experimental study on parenteral use of antibiotics for the treatment of bacterial diarrheal diseases by Ichinose Y. (1983)
10.1128/jcm.22.3.405-408.1985 / J. Clin. Microbiol. / New medium for the production of cholera toxin by Vibrio cholerae Ol biotype El Tor by Iwanaga M. (1985)
{'key': 'e_1_2_1_10_1', 'first-page': '80', 'article-title': 'Production of biologically active substances by two strains of Vibrio cholerae. Infect', 'volume': '1', 'author': 'Kusama H.', 'year': '1970', 'journal-title': 'Immun.'} / Immun. / Production of biologically active substances by two strains of Vibrio cholerae. Infect by Kusama H. (1970)
10.1111/j.1462-5822.2007.00901.x
10.1128/iai.20.2.552-558.1978 / Infect. Immun. / Purification of cholera toxin and its subunits: new method of preparation and the use of hypertoxigenic mutants by Mekalanos J.J. (1978)
10.1128/jb.100.1.27-34.1969 / Bacteriol. / Factors influencing in vitro skin permeability factor production by Vibrio cholerae. J by Richardson S.H. (1969)
10.11150/kansenshogakuzasshi1970.57.240
{'key': 'e_1_2_1_15_1', 'first-page': '501', 'article-title': 'Production of cholera toxin‐like toxin by Vibrio mimicus and non‐Ol Vibrio cholerae: batch culture conditions for optimum yields and isolation of hypertoxigenic lyncomycin‐resistant mutants. Infect', 'volume': '42', 'author': 'Spira W.M.', 'year': '1983', 'journal-title': 'Immun.'} / Immun. / Production of cholera toxin‐like toxin by Vibrio mimicus and non‐Ol Vibrio cholerae: batch culture conditions for optimum yields and isolation of hypertoxigenic lyncomycin‐resistant mutants. Infect by Spira W.M. (1983)
10.1128/iai.41.3.896-901.1983 / Infect. Immun. / Evidence that non‐Ol Vibrio cholerae produces enterotoxin that is similar but not identical to cholera enterotoxin by Yamamoto K. (1983)

Dates

Type	When
Created	11 years, 9 months ago (Nov. 14, 2013, 1:55 p.m.)
Deposited	1 year, 9 months ago (Nov. 20, 2023, 5:47 p.m.)
Indexed	9 hours, 20 minutes ago (Aug. 28, 2025, 8:17 a.m.)
Issued	38 years, 9 months ago (Nov. 1, 1986)
Published	38 years, 9 months ago (Nov. 1, 1986)
Published Online	11 years, 9 months ago (Nov. 14, 2013)
Published Print	38 years, 9 months ago (Nov. 1, 1986)

Funders 0

None

BibTeX

@article{Iwanaga_1986, title={Culture Conditions for Stimulating Cholera Toxin Production by Vibrio cholerae O1 El Tor}, volume={30}, ISSN={1348-0421}, url={http://dx.doi.org/10.1111/j.1348-0421.1986.tb03037.x}, DOI={10.1111/j.1348-0421.1986.tb03037.x}, number={11}, journal={Microbiology and Immunology}, publisher={Wiley}, author={Iwanaga, Masaaki and Yamamoto, Koichiro and Higa, Naomi and Ichinose, Yoshio and Nakasone, Noboru and Tanabe, Masao}, year={1986}, month=nov, pages={1075–1083} }

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