DOI: 10.1111/boc.201200060. FRET and FRAP imaging: Approaches to characterise tau and stathmin interactions with microtubules in cells

FRET and FRAP imaging: Approaches to characterise tau and stathmin interactions with microtubules in cells

10.1111/boc.201200060

Crossref journal-article

Wiley

Biology of the Cell (311)

Abstract

AbstractMicrotubules (MTs) are involved in many crucial processes such as cell morphogenesis, mitosis and motility. These dynamic structures resulting from the complex assembly of tubulin are tightly regulated by stabilising MT‐associated proteins (MAPs) such as tau and destabilising proteins, notably stathmin. Because of their key role, these MAPs and their interactions have been extensively studied using biochemical and biophysical approaches, particularly in vitro. Nevertheless, numerous questions remain unanswered and the mechanisms of interaction between MT and these proteins are still unclear in cells. Techniques coupling cell imaging and fluorescence methods, such as Förster resonance energy transfer and fluorescence recovery after photobleaching, are excellent tools to study these interactions in situ. After describing these methods, we will present emblematic data from the literature and unpublished experimental results from our laboratory concerning the interactions between MTs, tau and stathmin in cells.

Bibliography

Nouar, R., Devred, F., Breuzard, G., & Peyrot, V. (2013). FRET and FRAP imaging: Approaches to characterise tau and stathmin interactions with microtubules in cells. Biology of the Cell, 105(4), 149â161. Portico.

Authors 4

Roqiya Nouar (first)
François Devred (additional)
Gilles Breuzard (additional)
Vincent Peyrot (additional)

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Dates

Type	When
Created	12 years, 7 months ago (Jan. 14, 2013, 4:37 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:24 a.m.)
Indexed	1 month ago (July 30, 2025, 10:39 a.m.)
Issued	12 years, 6 months ago (Feb. 15, 2013)
Published	12 years, 6 months ago (Feb. 15, 2013)
Published Online	12 years, 6 months ago (Feb. 15, 2013)
Published Print	12 years, 5 months ago (April 1, 2013)

Funders 0

None

BibTeX

@article{Nouar_2013, title={FRET and FRAP imaging: Approaches to characterise tau and stathmin interactions with microtubules in cells}, volume={105}, ISSN={1768-322X}, url={http://dx.doi.org/10.1111/boc.201200060}, DOI={10.1111/boc.201200060}, number={4}, journal={Biology of the Cell}, publisher={Wiley}, author={Nouar, Roqiya and Devred, François and Breuzard, Gilles and Peyrot, Vincent}, year={2013}, month=feb, pages={149–161} }

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