DOI: 10.1111/1462-2920.12299. Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

10.1111/1462-2920.12299

Crossref journal-article

Wiley

Environmental Microbiology (311)

Abstract

SummaryChlamydiae comprise important pathogenic and symbiotic bacteria that alternate between morphologically and physiologically different life stages during their developmental cycle. Using electron cryotomography, we characterize the ultrastructure of the developmental stages of three environmental chlamydiae: Parachlamydia acanthamoebae, Protochlamydia amoebophila and Simkania negevensis. We show that chemical fixation and dehydration alter the cell shape of Parachlamydia and that the crescent body is not a developmental stage, but an artefact of conventional electron microscopy. We further reveal type III secretion systems of environmental chlamydiae at macromolecular resolution and find support for a chlamydial needle‐tip protein. Imaging bacteria inside their host cells by cryotomography for the first time, we observe marked differences in inclusion morphology and development as well as host organelle recruitment between the three chlamydial organisms, with Simkania inclusions being tightly enveloped by the host endoplasmic reticulum. The study demonstrates the power of electron cryotomography to reveal structural details of bacteria–host interactions that are not accessible using traditional methods.

Bibliography

Pilhofer, M., Aistleitner, K., Ladinsky, M. S., KÃ¶nig, L., Horn, M., & Jensen, G. J. (2013). Architecture and host interface of environmental chlamydiae revealed by electron cryotomography. Environmental Microbiology, 16(2), 417â429. Portico.

Authors 6

Martin Pilhofer (first)
Karin Aistleitner (additional)
Mark S. Ladinsky (additional)
Lena König (additional)
Matthias Horn (additional)
Grant J. Jensen (additional)

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Dates

Type	When
Created	11 years, 10 months ago (Oct. 11, 2013, 10:09 p.m.)
Deposited	1 year, 10 months ago (Sept. 24, 2023, 10:04 p.m.)
Indexed	1 month ago (July 22, 2025, 6:56 a.m.)
Issued	11 years, 9 months ago (Nov. 8, 2013)
Published	11 years, 9 months ago (Nov. 8, 2013)
Published Online	11 years, 9 months ago (Nov. 8, 2013)
Published Print	11 years, 6 months ago (Feb. 1, 2014)

Funders 0

None

BibTeX

@article{Pilhofer_2013, title={Architecture and host interface of environmental chlamydiae revealed by electron cryotomography}, volume={16}, ISSN={1462-2920}, url={http://dx.doi.org/10.1111/1462-2920.12299}, DOI={10.1111/1462-2920.12299}, number={2}, journal={Environmental Microbiology}, publisher={Wiley}, author={Pilhofer, Martin and Aistleitner, Karin and Ladinsky, Mark S. and König, Lena and Horn, Matthias and Jensen, Grant J.}, year={2013}, month=nov, pages={417–429} }

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