DOI: 10.1111/1365-3040.ep11591840. Salt‐tolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes

Salt‐tolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes

10.1111/1365-3040.ep11591840

Crossref journal-article

Wiley

Plant, Cell & Environment (311)

Abstract

Abstract Polysomes and ribosomes recovered from a number of plant species were tested for stability when incubated at 25°C in salt solutions in the absence of ATP and initiation factors. Stability was assessed by sucrose density gradient analysis. The stability was inversely proportional to salt concentrations above 125 mol m−3 KCl. Polysomes were less stable in the presence of Na+ than K+ salts, and were much less stable in Cl− than in acetate salts. Polysomes from Triticum aestivum. Hordeum vulgare, Capsicum annuum, Helianthus annuus. Pisum sativum, Atriplex nummularia, Beta vulgaris, Cladophora sp., Enteromorpha sp. and Corallina cuvieri were similarly sensitive to KCl. Polysomes from Ulva lactuca were more sensitive than the other species. Cytoplasmic and plastid polysomes from T. aestivum were similarly unstable in 500 mol m−3 KCl. Unprogrammed ribosomal subunit couples from T. aestivum, B. vulgaris and U. lactuca showed Mg2+‐dependent conformational instability and dissociation in KCl. Slight differences in ribosomal stability were observed between species, but these were unrelated to the salt tolerances of the plants. The ‘compatible’ organic solutes, glycinebetaine and proline, failed to reduce ion‐induced instability. Ribosome yield and polysome profiles were similar in leaves of B. vulgaris containing significantly different levels of both Na+ and Cl− after growth in media containing 50 or 200 mol m−3 NaCl. The results are consistent with the hypothesis that plants maintain a cytoplasmic solute environment that is compatible with ribosomal stability.

Bibliography

BRADY, C. J., GIBSON, T. S., BARLOW, E. W. R., SPEIRS, J., & WYN JONES, R. G. (1984). Saltâtolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes. Plant, Cell & Environment, 7(8), 571â578. Portico.

Authors 5

C. J. BRADY (first)
T. S. GIBSON (additional)
E. W. R. BARLOW (additional)
J. SPEIRS (additional)
R. G. WYN JONES (additional)

References 28 Referenced 10

10.1093/jxb/28.4.909
10.1071/PP9770327
10.1099/00221287-72-3-589
10.1016/0005-2787(72)90158-X
10.1016/0005-2787(73)90321-3
10.1016/0079-6107(78)90020-2
10.1104/pp.50.5.581
10.1071/BI9731251 / Australian Journal of Biological Sciences / Effect of light on the volume and ion relations of chloroplasts in detached leaves of Elodea densa by Filippis L.F. (1973)
10.1146/annurev.pp.28.060177.000513
10.1111/1365-3040.ep11591845
10.1007/BF00397406
10.1146/annurev.pp.31.060180.001053
10.1007/BF01276291
10.1007/BF00387435
10.1016/0005-2736(70)90043-X
10.1085/jgp.47.5.859
10.1016/S0021-9258(18)63264-8 / Journal of Biological Chemistry / Resistance of active yeast ribosomes to dissociation by KCl by Martin T.E. (1970)
10.1016/0022-2836(69)90084-9
10.1016/S0031-9422(00)88945-1
10.1007/BF00388772
10.1083/jcb.44.2.400
10.1021/bi00547a001
10.1111/j.1432-1033.1977.tb11263.x
{'key': 'e_1_2_1_25_1', 'first-page': '1124', 'volume-title': 'Methods of Soil Analysis', 'author': 'Stout P.R.', 'year': '1965'} / Methods of Soil Analysis by Stout P.R. (1965)
10.1021/bi00764a025
10.1016/0005-2787(72)90105-0
10.1111/j.1432-1033.1972.tb01822.x
{'key': 'e_1_2_1_29_1', 'first-page': '121', 'volume-title': 'Regulation of Cell Membrane Activities in Plants', 'author': 'Wyn Jones R.G.', 'year': '1977'} / Regulation of Cell Membrane Activities in Plants by Wyn Jones R.G. (1977)

Dates

Type	When
Created	19 years, 4 months ago (April 30, 2006, 4:23 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 8:37 a.m.)
Indexed	1 year, 10 months ago (Oct. 27, 2023, 6:02 p.m.)
Issued	40 years, 10 months ago (Nov. 1, 1984)
Published	40 years, 10 months ago (Nov. 1, 1984)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	40 years, 10 months ago (Nov. 1, 1984)

Funders 0

None

BibTeX

@article{BRADY_1984, title={Salt‐tolerance in plants. I. Ions, compatible organic solutes and the stability of plant ribosomes}, volume={7}, ISSN={1365-3040}, url={http://dx.doi.org/10.1111/1365-3040.ep11591840}, DOI={10.1111/1365-3040.ep11591840}, number={8}, journal={Plant, Cell & Environment}, publisher={Wiley}, author={BRADY, C. J. and GIBSON, T. S. and BARLOW, E. W. R. and SPEIRS, J. and WYN JONES, R. G.}, year={1984}, month=nov, pages={571–578} }

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