DOI: 10.1110/ps.9.12.2329. Structural comparison of Ntn‐hydrolases

10.1110/ps.9.12.2329

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe Ntn‐hydrolases (N‐terminal nucleophile) are a superfamily of diverse enzymes that has recently been characterized. All of the proteins in this family are activated autocatalytically; they contain an N‐terminally located catalytic nucleophile, and they cleave an amide bond. In the present study, the structures of four enzymes of this superfamily are compared in more detail. Although the amino acid sequence homology is almost completely absent, the enzymes share a similar αββα‐core structure. The central β‐sheets in the core were found to have different packing angles, ranging from 5 to 35°. In the Ntn‐hydrolases under study, eight totally conserved secondary structure units were found (region C). Five of them were observed to contain the greatest number of conserved and functionally important residues and are therefore crucial for the structure and function of Ntn‐hydrolases. Two additional regions, consisting of secondary structure units (regions A and B), were found to be in structurally similar locations, but in different orders in the polypeptide chain. The catalytic machinery is located in the structures in a similar manner, and thus the catalytic mechanisms of all of the enzymes are probably similar. However, the substrate binding and the oxyanion hole differed partially.

Bibliography

Oinonen, C., & Rouvinen, J. (2000). Structural comparison of Ntnâhydrolases. Protein Science, 9(12), 2329â2337. Portico.

Authors 2

Carita Oinonen (first)
Juha Rouvinen (additional)

References 28 Referenced 216

10.1038/nsb1295-1035
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10.1016/S0969-2126(00)00091-5
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Dates

Type	When
Created	16 years, 6 months ago (Feb. 17, 2009, 5:49 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 2:44 p.m.)
Indexed	3 months, 3 weeks ago (May 6, 2025, 7:25 a.m.)
Issued	25 years, 8 months ago (Jan. 1, 2000)
Published	25 years, 8 months ago (Jan. 1, 2000)
Published Online	16 years, 8 months ago (Dec. 31, 2008)
Published Print	25 years, 8 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Oinonen_2000, title={Structural comparison of Ntn‐hydrolases}, volume={9}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.9.12.2329}, DOI={10.1110/ps.9.12.2329}, number={12}, journal={Protein Science}, publisher={Wiley}, author={Oinonen, Carita and Rouvinen, Juha}, year={2000}, month=jan, pages={2329–2337} }

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