DOI: 10.1110/ps.9.10.1960. Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy

Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy

10.1110/ps.9.10.1960

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractFourier transform infrared spectroscopy (FTIR), circular dichroism (CD), and electron microscopy (EM) have been used simultaneously to follow the temperature‐induced formation of amyloid fibrils by bovine insulin at acidic pH. The FTIR and CD data confirm that, before heating, insulin molecules in solution at pH 2.3 have a predominantly native‐like α‐helical structure. On heating to 70°C, partial unfolding occurs and results initially in aggregates that are shown by CD and FT‐IR spectra to retain a predominantly helical structure. Following this step, changes in the CD and FTIR spectra occur that are indicative of the extensive conversion of the molecular conformation from α‐helical to β‐sheet structure. At later stages, EM shows the development of fibrils with well‐defined repetitive morphologies including structures with a periodic helical twist of ∼450 Å. The results indicate that formation of fibrils by insulin requires substantial unfolding of the native protein, and that the most highly ordered structures result from a slow evolution of the morphology of the initially formed fibrillar species.

Bibliography

Bouchard, M., Zurdo, J., Nettleton, E. J., Dobson, C. M., & Robinson, C. V. (2000). Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy. Protein Science, 9(10), 1960â1967. Portico.

Authors 5

Mario Bouchard (first)
Jesús Zurdo (additional)
Ewan J. Nettleton (additional)
Christopher M. Dobson (additional)
Carol V. Robinson (additional)

References 48 Referenced 426

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{'key': 'e_1_2_1_33_1', 'first-page': '1512', 'volume-title': 'The Oxford textbook of medicine', 'author': 'Pepys MB', 'year': '1996'} / The Oxford textbook of medicine by Pepys MB (1996)
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Dates

Type	When
Created	16 years, 6 months ago (Feb. 17, 2009, 5:32 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 1:36 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 3, 2025, 12:30 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Bouchard_2000, title={Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy}, volume={9}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.9.10.1960}, DOI={10.1110/ps.9.10.1960}, number={10}, journal={Protein Science}, publisher={Wiley}, author={Bouchard, Mario and Zurdo, Jesús and Nettleton, Ewan J. and Dobson, Christopher M. and Robinson, Carol V.}, year={2000}, month=jan, pages={1960–1967} }

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