DOI: 10.1110/ps.8.4.854. De novo design of a monomeric three‐stranded antiparallel β‐sheet

De novo design of a monomeric three‐stranded antiparallel β‐sheet

10.1110/ps.8.4.854

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractHere we describe the NMR conformational study of a 20‐residue linear peptide designed to fold into a monomeric three‐stranded antiparallel β‐sheet in aqueous solution. Experimental and statistical data on amino acid β‐turn and β‐sheet propensities, cross‐strand side‐chain interactions, solubility criteria, and our previous experience with β‐hairpins were considered for a rational selection of the peptide sequence. Sedimentation equilibrium measurements and NMR dilution experiments provide evidence that the peptide is monomeric. Analysis of 1H and 13C‐NMR parameters of the peptide, in particular NOEs and chemical shifts, and comparison with data obtained for two 12‐residue peptides encompassing the N‐ and C‐segments of the designed sequence indicates that the 20‐residue peptide folds into the expected conformation. Assuming a two‐state model, the exchange kinetics between the β‐sheet and the unfolded peptide molecules is in a suitable range to estimate the folding rate on the basis of the NMR linewidths of several resonances. The time constant for the coil‐β‐sheet transition is of the order of several microseconds in the designed peptide. Future designs based on this peptide system are expected to contribute greatly to our knowledge of the many factors involved in β‐sheet formation and stability.

Bibliography

Alba, E. D., Santoro, J., Rico, M., & JimÃ©nez, M. A. (1999). De novo design of a monomeric threeâstranded antiparallel Î²âsheet. Protein Science, 8(4), 854â865. Portico.

Authors 4

Eva De Alba (first)
Jorge Santoro (additional)
Manuel Rico (additional)
M. Angeles Jiménez (additional)

References 77 Referenced 122

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Dates

Type	When
Created	14 years, 11 months ago (Oct. 1, 2010, 9:44 a.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 3:04 p.m.)
Indexed	1 month ago (July 30, 2025, 10:52 a.m.)
Issued	26 years, 8 months ago (Jan. 1, 1999)
Published	26 years, 8 months ago (Jan. 1, 1999)
Published Online	16 years, 8 months ago (Dec. 31, 2008)
Published Print	26 years, 8 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Alba_1999, title={De novo design of a monomeric three‐stranded antiparallel β‐sheet}, volume={8}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.8.4.854}, DOI={10.1110/ps.8.4.854}, number={4}, journal={Protein Science}, publisher={Wiley}, author={Alba, Eva De and Santoro, Jorge and Rico, Manuel and Jiménez, M. Angeles}, year={1999}, month=jan, pages={854–865} }

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