DOI: 10.1110/ps.8.11.2428. A test case for structure‐based functional assignment: The 1.2 Å crystal structure of the yjgF gene product from <i>Escherichia coli</i>

A test case for structure‐based functional assignment: The 1.2 Å crystal structure of the yjgF gene product from Escherichia coli

10.1110/ps.8.11.2428

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe YER057c/YIL051c/YjgF protein family is a set of 24 full‐length homologs, each ∼130 residues in length, and each with no known function or relationship to proteins of known structure. To determine the function of this family, the structure of one member–the YjgF protein from Escherichia coli–was solved and refined at a resolution of 1.2 Å. The YjgF molecule is a homotrimer with exact threefold symmetry. Its tertiary and quaternary structures are related to that of Bacillus subtilis chorismate mutase, although their active sites are completely different. The YjgF protein has an active site curiously similar to protein tyrosine phosphatases, including a covalently modified cysteine, but it is unlikely to be functionally related. The lessons learned from this attempt to deduce function from structure may be useful to future projects in structural genomics.

Bibliography

Volz, K. (1999). A test case for structureâbased functional assignment: The 1.2 Ã crystal structure of the yjgF gene product from Escherichia coli. Protein Science, 8(11), 2428â2437. Portico.

Authors 1

Karl Volz (first)

References 46 Referenced 70

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Dates

Type	When
Created	16 years, 6 months ago (Feb. 17, 2009, 4:37 p.m.)
Deposited	1 year, 9 months ago (Oct. 28, 2023, 5:44 p.m.)
Indexed	4 weeks, 1 day ago (July 28, 2025, 5:06 p.m.)
Issued	26 years, 7 months ago (Jan. 1, 1999)
Published	26 years, 7 months ago (Jan. 1, 1999)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	26 years, 7 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Volz_1999, title={A test case for structure‐based functional assignment: The 1.2 Å crystal structure of the yjgF gene product from Escherichia coli}, volume={8}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.8.11.2428}, DOI={10.1110/ps.8.11.2428}, number={11}, journal={Protein Science}, publisher={Wiley}, author={Volz, Karl}, year={1999}, month=jan, pages={2428–2437} }

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