DOI: 10.1110/ps.33601. Solvation energetics and conformational change in EF‐hand proteins

Solvation energetics and conformational change in EF‐hand proteins

10.1110/ps.33601

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractCalmodulin and other members of the EF‐hand protein family are known to undergo major changes in conformation upon binding Ca2+. However, some EF‐hand proteins, such as calbindin D9k, bind Ca2+ without a significant change in conformation. Here, we show the importance of a precise balance of solvation energetics to conformational change, using mutational analysis of partially buried polar groups in the N‐terminal domain of calmodulin (N‐cam). Several variants were characterized using fluorescence, circular dichroism, and NMR spectroscopy. Strikingly, the replacement of polar side chains glutamine and lysine at positions 41 and 75 with nonpolar side chains leads to dramatic enhancement of the stability of the Ca2+‐free state, a corresponding decrease in Ca2+‐binding affinity, and an apparent loss of ability to change conformation to the open form. The results suggest a paradigm for conformational change in which energetic strain is accumulated in one state in order to modulate the energetics of change to the alternative state.

Bibliography

Ababou, A., & Desjarlais, J. R. (2001). Solvation energetics and conformational change in EFâhand proteins. Protein Science, 10(2), 301â312. Portico.

Authors 2

Abdessamad Ababou (first)
John R. Desjarlais (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 3:11 a.m.)
Indexed	40 minutes ago (Aug. 29, 2025, 6:30 a.m.)
Issued	24 years, 6 months ago (Feb. 1, 2001)
Published	24 years, 6 months ago (Feb. 1, 2001)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	24 years, 6 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Ababou_2001, title={Solvation energetics and conformational change in EF‐hand proteins}, volume={10}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.33601}, DOI={10.1110/ps.33601}, number={2}, journal={Protein Science}, publisher={Wiley}, author={Ababou, Abdessamad and Desjarlais, John R.}, year={2001}, month=feb, pages={301–312} }

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