DOI: 10.1110/ps.17901. Structure of the transmembrane region of the M2 protein H<sup>+</sup> channel

Structure of the transmembrane region of the M2 protein H+ channel

10.1110/ps.17901

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe transmembrane domain of the M2 protein from influenza A virus forms a nearly uniform and ideal helix in a liquid crystalline bilayer environment. The exposure of the hydrophilic backbone structure is minimized through uniform hydrogen bond geometry imposed by the low dielectric lipid environment. A high‐resolution structure of the monomer backbone and a detailed description of its orientation with respect to the bilayer were achieved using orientational restraints from solid‐state NMR. With this unique information, the tetrameric structure of this H+ channel is constrained substantially. Features of numerous published models are discussed in light of the experimental structure of the monomer and derived features of the tetrameric bundle.

Bibliography

Wang, J., Kim, S., Kovacs, F., & Cross, T. A. (2001). Structure of the transmembrane region of the M2 protein H+ channel. Protein Science, 10(11), 2241â2250. Portico.

Authors 4

Junfeng Wang (first)
Sanguk Kim (additional)
Frank Kovacs (additional)
Timothy A. Cross (additional)

References 44 Referenced 213

10.1073/pnas.93.12.5872
10.1002/jcc.540040211
10.1021/bi982981m
10.1016/0042-6822(92)91239-Q
10.1006/viro.1994.1345
10.1016/0014-5793(92)81114-2
10.1126/science.274.5294.1850
10.1111/j.1399-3011.1989.tb01285.x
10.1073/pnas.85.5.1384
10.1016/S0006-3495(00)76572-6
10.1016/0022-2836(94)90063-9
10.1016/0042-6822(91)90115-R
10.1021/bi970146j
{'key': 'e_1_2_6_15_1', 'volume-title': 'Hydrogen Bonding in Biological Systems', 'author': 'Jeffrey G.A.', 'year': '1994'} / Hydrogen Bonding in Biological Systems by Jeffrey G.A. (1994)
10.1126/science.7690158
{'key': 'e_1_2_6_17_1', 'first-page': '299', 'volume-title': 'Membrane Structure and Dynamics', 'author': 'Ketchem R.R.', 'year': '1996'} / Membrane Structure and Dynamics by Ketchem R.R. (1996)
10.1016/S0969-2126(97)00312-2
10.1126/science.220.4598.671
10.1146/annurev.bb.25.060196.001311
10.1016/S0006-3495(97)78279-1
10.1006/jmbi.1999.3322
10.1006/jmbi.1998.2512
{'key': 'e_1_2_6_24_1', 'first-page': '303', 'volume-title': 'Receptor‐Mediated Virus Entry into Cell', 'author': 'Lamb R.A.', 'year': '1994'} / Receptor‐Mediated Virus Entry into Cell by Lamb R.A. (1994)
10.1006/jmre.2000.2035
10.1063/1.1699114
10.1002/bip.1980.360190605
10.1016/S0006-3495(99)77085-2
10.1073/pnas.94.21.11301
10.1016/S0006-3495(97)78878-7
10.1002/(SICI)1099-0534(2000)12:2<71::AID-CMR2>3.0.CO;2-R
10.1073/pnas.94.10.5000
{'key': 'e_1_2_6_33_1', 'first-page': 'A123', 'article-title': 'Aggregation of influenza A M2 transmembrane segment in micelles', 'volume': '76', 'author': 'Salom D.', 'year': '1999', 'journal-title': 'Biophys. J.'} / Biophys. J. / Aggregation of influenza A M2 transmembrane segment in micelles by Salom D. (1999)
10.1016/S0006-3495(67)86579-2
10.1016/S0006-3495(00)76581-7
10.1016/S0006-3495(00)76334-X
10.1021/ja00039a048
10.1128/JVI.73.12.9695-9701.1999 / J. Virol. / Effects of cytoplasmic tail truncations on the activity of the 2 ion channel of influenza A virus by Tobler K. (1999)
{'key': 'e_1_2_6_39_1', 'volume-title': 'Biochemistry', 'author': 'Voet D.', 'year': '1990'} / Biochemistry by Voet D. (1990)
10.1128/jvi.67.9.5585-5594.1993 / J. Virol. / Ion channel activity of the influenza A virus M2 protein: Characterization of the amantadine block by Wang C. (1993)
10.1006/jmre.2000.2037
10.1006/jmra.1994.1169
10.1073/pnas.96.16.9057
10.1016/S0014-5793(98)00988-0
10.1038/81919

Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 8, 2023, 8:24 a.m.)
Indexed	1 month ago (July 30, 2025, 10:15 a.m.)
Issued	23 years, 10 months ago (Nov. 1, 2001)
Published	23 years, 10 months ago (Nov. 1, 2001)
Published Online	16 years, 8 months ago (Dec. 31, 2008)
Published Print	23 years, 10 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Wang_2001, title={Structure of the transmembrane region of the M2 protein H+ channel}, volume={10}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.17901}, DOI={10.1110/ps.17901}, number={11}, journal={Protein Science}, publisher={Wiley}, author={Wang, Junfeng and Kim, Sanguk and Kovacs, Frank and Cross, Timothy A.}, year={2001}, month=nov, pages={2241–2250} }

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