DOI: 10.1110/ps.073389408. NMR solution structure of KP‐TerB, a tellurite‐resistance protein from <i>Klebsiella pneumoniae</i>

NMR solution structure of KP‐TerB, a tellurite‐resistance protein from Klebsiella pneumoniae

10.1110/ps.073389408

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractKlebsiella pneumoniae (KP), a Gram‐negative bacterium, is a common cause of hospital‐acquired bacterial infections worldwide. Tellurium (Te) compounds, although relatively rare in the environment, have a long history as antimicrobial and therapeutic agents. In bacteria, tellurite (TeO3 −2) resistance is conferred by the ter (Ter) operon (terZABCDEF). Here, on the basis of 2593 restraints derived from NMR analysis, we report the NMR structure of TerB protein (151 amino acids) of KP (KP‐TerB), which is mainly composed of seven α‐helices and a 310 helix, with helices II to V apparently forming a four‐helix bundle. The ensemble of 20 NMR structures was well‐defined, with a RMSD of 0.32 ± 0.06 Å for backbone atoms and 1.11 ± 0.07 Å for heavy atoms, respectively. A unique property of the KP‐TerB structure is that the positively and negatively charged clusters are formed by the N‐terminal positively and C‐terminal negatively charged residues, respectively. To the best of our knowledge, the protein sequence and structures of KP‐TerB are unique.

Bibliography

Chiang, S., Lou, Y., & Chen, C. (2008). NMR solution structure of KPâTerB, a telluriteâresistance protein from Klebsiella pneumoniae. Protein Science, 17(4), 785â789. Portico.

Authors 3

Sheng‐Kuo Chiang (first)
Yuan‐Chao Lou (additional)
Chinpan Chen (additional)

References 21 Referenced 16

Dates

Type	When
Created	17 years, 6 months ago (Feb. 27, 2008, 9:39 p.m.)
Deposited	1 year, 11 months ago (Sept. 29, 2023, 12:52 a.m.)
Indexed	1 year, 1 month ago (July 23, 2024, 10:21 p.m.)
Issued	17 years, 5 months ago (April 1, 2008)
Published	17 years, 5 months ago (April 1, 2008)
Published Online	16 years, 8 months ago (Jan. 2, 2009)
Published Print	17 years, 5 months ago (April 1, 2008)

Funders 0

None

BibTeX

@article{Chiang_2008, title={NMR solution structure of KP‐TerB, a tellurite‐resistance protein from Klebsiella pneumoniae}, volume={17}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.073389408}, DOI={10.1110/ps.073389408}, number={4}, journal={Protein Science}, publisher={Wiley}, author={Chiang, Sheng‐Kuo and Lou, Yuan‐Chao and Chen, Chinpan}, year={2008}, month=apr, pages={785–789} }

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