DOI: 10.1110/ps.073174008. Structural determinants of nitroxide motion in spin‐labeled proteins: Solvent‐exposed sites in helix B of T4 lysozyme

Structural determinants of nitroxide motion in spin‐labeled proteins: Solvent‐exposed sites in helix B of T4 lysozyme

10.1110/ps.073174008

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractSite‐directed spin labeling provides a means for exploring structure and dynamics in proteins. To interpret the complex EPR spectra that often arise, it is necessary to characterize the rotamers of the spin‐labeled side chain and the interactions they make with the local environment in proteins of known structure. For this purpose, crystal structures have been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the solvent‐exposed helical sites 41 and 44 in the B helix. These sites are of particular interest in that the corresponding EPR spectra reveal two dynamic states of R1, one of which is relatively immobilized suggesting interactions of the nitroxide with the environment. The crystal structures together with the effect of mutagenesis of nearest neighbors on the motion of R1 suggest intrahelical interactions of 41R1 with the i + 4 residue and of 44R1 with the i + 1 residue. Such interactions appear to be specific to particular rotamers of the R1 side chain.

Bibliography

Guo, Z., Cascio, D., Hideg, K., & Hubbell, W. L. (2008). Structural determinants of nitroxide motion in spinâlabeled proteins: Solventâexposed sites in helix B of T4 lysozyme. Protein Science, 17(2), 228â239. Portico.

Authors 4

Zhefeng Guo (first)
Duilio Cascio (additional)
Kálmán Hideg (additional)
Wayne L. Hubbell (additional)

References 42 Referenced 112

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Dates

Type	When
Created	17 years, 8 months ago (Dec. 20, 2007, 8:59 p.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 6:45 p.m.)
Indexed	4 weeks ago (July 30, 2025, 10:57 a.m.)
Issued	17 years, 6 months ago (Feb. 1, 2008)
Published	17 years, 6 months ago (Feb. 1, 2008)
Published Online	16 years, 7 months ago (Jan. 2, 2009)
Published Print	17 years, 6 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Guo_2008, title={Structural determinants of nitroxide motion in spin‐labeled proteins: Solvent‐exposed sites in helix B of T4 lysozyme}, volume={17}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.073174008}, DOI={10.1110/ps.073174008}, number={2}, journal={Protein Science}, publisher={Wiley}, author={Guo, Zhefeng and Cascio, Duilio and Hideg, Kálmán and Hubbell, Wayne L.}, year={2008}, month=feb, pages={228–239} }

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