DOI: 10.1110/ps.04864904. Crystal structure of ribosomal protein L27 from <i>Thermus thermophilus</i> HB8

Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8

10.1110/ps.04864904

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractRibosomal protein L27 is located near the peptidyltransferase center at the interface of ribosomal subunits, and is important for ribosomal assembly and function. We report the crystal structure of ribosomal protein L27 from Thermus thermophilus HB8, which was determined by the multiwavelength anomalous dispersion method and refined to an R‐factor of 19.7% (Rfree = 23.6%) at 2.8 Å resolution. The overall fold is an all β‐sheet hybrid. It consists of two sets of four‐stranded β‐sheets formed around a well‐defined hydrophobic core, with a highly positive charge on the protein surface. The structure of ribosomal protein L27 from T. thermophilus HB8 in the RNA‐free form is investigated, and its functional roles in the ribosomal subunit are discussed.

Bibliography

Wang, H., Takemoto, C. H., Murayama, K., Sakai, H., Tatsuguchi, A., Terada, T., Shirouzu, M., Kuramitsu, S., & Yokoyama, S. (2004). Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8. Protein Science, 13(10), 2806â2810. Portico.

Authors 9

Hongfei Wang (first)
Chie Hori Takemoto (additional)
Kazutaka Murayama (additional)
Hiroaki Sakai (additional)
Ayako Tatsuguchi (additional)
Takaho Terada (additional)
Mikako Shirouzu (additional)
Seiki Kuramitsu (additional)
Shigeyuki Yokoyama (additional)

References 32 Referenced 9

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{'key': 'e_1_2_6_24_1', 'first-page': '123', 'volume-title': 'The ribosome: Structure, function and evolution', 'author': 'Stöffler‐Meilicke M.', 'year': '1990'} / The ribosome: Structure, function and evolution by Stöffler‐Meilicke M. (1990)
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Dates

Type	When
Created	20 years, 11 months ago (Aug. 31, 2004, 8:24 p.m.)
Deposited	1 year, 10 months ago (Oct. 9, 2023, 3:14 p.m.)
Indexed	1 year, 3 months ago (May 11, 2024, 3:44 a.m.)
Issued	20 years, 10 months ago (Oct. 1, 2004)
Published	20 years, 10 months ago (Oct. 1, 2004)
Published Online	16 years, 7 months ago (Jan. 1, 2009)
Published Print	20 years, 10 months ago (Oct. 1, 2004)

Funders 0

None

BibTeX

@article{Wang_2004, title={Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8}, volume={13}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.04864904}, DOI={10.1110/ps.04864904}, number={10}, journal={Protein Science}, publisher={Wiley}, author={Wang, Hongfei and Takemoto, Chie Hori and Murayama, Kazutaka and Sakai, Hiroaki and Tatsuguchi, Ayako and Terada, Takaho and Shirouzu, Mikako and Kuramitsu, Seiki and Yokoyama, Shigeyuki}, year={2004}, month=oct, pages={2806–2810} }

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