DOI: 10.1110/ps.041087605. Helical structure determined by NMR of the HIV‐1 (345–392)Gag sequence, surrounding p2: Implications for particle assembly and RNA packaging

Helical structure determined by NMR of the HIV‐1 (345–392)Gag sequence, surrounding p2: Implications for particle assembly and RNA packaging

10.1110/ps.041087605

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractGag protein oligomerization, an essential step during virus assembly, results in budding of spherical virus particles. This process is critically dependent on the spacer p2, located between the capsid and the nucleocapsid proteins. P2 contributes also, in association with NCp7, to specific recognition of the HIV‐1 packaging signal resulting in viral genome encapsidation. There is no structural information about the 20 last amino acids of the C‐terminal part of capsid (CA[CTD]) and p2, in the molecular mechanism of Gag assembly. In this study the structure of a peptide encompassing the 14 residues of p2 with the upstream 21 residues and the downstream 13 residues was determined by 1H NMR in 30% trifluoroethanol (TFE). The main structural motif is a well‐defined amphipathic α‐helix including p2, the seven last residues of the CA(CTD), and the two first residues of NCp7. Peptides containing the p2 domain have a strong tendency to aggregate in solution, as shown by gel filtration analyses in pure H2O. To take into account the aggregation phenomena, models of dimer and trimer formed through hydrophobic or hydrophilic interfaces were constructed by molecular dynamic simulations. Gel shift experiments demonstrate that the presence of at least p2 and the 13 first residues of NCp7 is required for RNA binding. A computer‐generated model of the Gag polyprotein segment (282–434)Gag interacting with the packaging element SL3 is proposed, illustrating the importance of p2 and NCp7 in genomic encapsidation.

Bibliography

Morellet, N., Druillennec, S., Lenoir, C., Bouaziz, S., & Roques, B. P. (2005). Helical structure determined by NMR of the HIVâ1 (345â392)Gag sequence, surrounding p2: Implications for particle assembly and RNA packaging. Protein Science, 14(2), 375â386. Portico.

Authors 5

Nelly Morellet (first)
Sabine Druillennec (additional)
Christine Lenoir (additional)
Serge Bouaziz (additional)
Bernard P. Roques (additional)

References 63 Referenced 74

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Dates

Type	When
Created	20 years, 7 months ago (Jan. 18, 2005, 11:27 p.m.)
Deposited	1 year, 10 months ago (Oct. 12, 2023, 8:30 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 6, 2024, 1:43 p.m.)
Issued	20 years, 7 months ago (Feb. 1, 2005)
Published	20 years, 7 months ago (Feb. 1, 2005)
Published Online	16 years, 8 months ago (Jan. 1, 2009)
Published Print	20 years, 7 months ago (Feb. 1, 2005)

Funders 0

None

BibTeX

@article{Morellet_2005, title={Helical structure determined by NMR of the HIV‐1 (345–392)Gag sequence, surrounding p2: Implications for particle assembly and RNA packaging}, volume={14}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.041087605}, DOI={10.1110/ps.041087605}, number={2}, journal={Protein Science}, publisher={Wiley}, author={Morellet, Nelly and Druillennec, Sabine and Lenoir, Christine and Bouaziz, Serge and Roques, Bernard P.}, year={2005}, month=feb, pages={375–386} }

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