DOI: 10.1110/ps.03267503. Self‐assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers

Self‐assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers

10.1110/ps.03267503

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractOne of the biggest challenges in pharmaceutical research is obtaining integral membrane proteins in a functional, solubilized, and monodisperse state that provides a native‐like environment that maintains the spectrum of in vivo activities. Many of these integral membrane proteins are receptors, enzymes, or other macromolecular assemblies that are important drug targets. An example is the general class of proteins composed of seven‐transmembrane segments (7‐TM) as exemplified by the G‐protein–coupled receptors. In this article, we describe a simple system for self‐assembling bacteriorhodopsin, as a model protein containing 7‐TM helices, with phospholipids to form a nanometer‐scale soluble bilayer structure encircled by a 200 amino acid scaffold protein. The result is the single molecule incorporation of an integral membrane protein target into a soluble and monodisperse structure that allows the structural and functional tools of solution biochemistry to be applied.

Bibliography

Bayburt, T. H., & Sligar, S. G. (2003). Selfâassembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers. Protein Science, 12(11), 2476â2481. Portico.

Authors 2

Timothy H. Bayburt (first)
Stephen G. Sligar (additional)

References 26 Referenced 232

10.1111/j.1432-1033.1976.tb10334.x
10.1016/S0006-3495(79)85271-6
10.1021/nl025623k
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10.1021/bi00297a027
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10.1016/0014-5793(78)80427-X
10.1016/0076-6879(82)88005-1
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{'key': 'e_1_2_6_23_1', 'first-page': '877', 'article-title': 'Properties of lipid•apolipoprotein association products', 'volume': '255', 'author': 'Swaney J.B.', 'year': '1980', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Properties of lipid•apolipoprotein association products by Swaney J.B. (1980)
10.1016/0301-4622(86)85069-4
10.1016/S0006-3495(84)84072-2
10.1021/la001689o
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Dates

Type	When
Created	21 years, 10 months ago (Oct. 22, 2003, 3:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 8, 2023, 9:54 a.m.)
Indexed	4 weeks, 1 day ago (July 30, 2025, 11:13 a.m.)
Issued	21 years, 9 months ago (Nov. 1, 2003)
Published	21 years, 9 months ago (Nov. 1, 2003)
Published Online	16 years, 7 months ago (Jan. 1, 2009)
Published Print	21 years, 9 months ago (Nov. 1, 2003)

Funders 0

None

BibTeX

@article{Bayburt_2003, title={Self‐assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers}, volume={12}, ISSN={1469-896X}, url={http://dx.doi.org/10.1110/ps.03267503}, DOI={10.1110/ps.03267503}, number={11}, journal={Protein Science}, publisher={Wiley}, author={Bayburt, Timothy H. and Sligar, Stephen G.}, year={2003}, month=nov, pages={2476–2481} }

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