DOI: 10.1104/pp.126.4.1630. Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants

Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants

10.1104/pp.126.4.1630

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract Nucleotide positions conserved on the 3′ side of the initiator codon ATG and the corresponding N-terminal amino acid residues in a number of highly abundant plant proteins were identified by computational analysis of a dataset of highly expressed plant genes. The reporter genes uidA and gfp were modified to introduce these features. Insertion of GCT TCC TCC after the initiator codon ATG augmented expression for both the reporter genes. The insertion of each successive codon improved the expression of β-glucuronidase (GUS) in an incremental fashion in transient transformation of tobacco (Nicotiana tabacum) leaves. The insertion of alanine-serine (Ser)-Ser resulted in about a 2-fold increase in the stability of GUS. However, this did not account for the 30- to 40-fold increase in GUS activity between the constructs coding for methionine-alanine-Ser-Ser-GUS and the native enzyme. Substitution of the codon for Ser at the third amino acid residue with synonymous codons reduced GUS expression. The results suggest a role for the conserved nucleotides in the +4 to +11 region in augmenting posttranscriptional events in the expression of genes in plants.

Bibliography

Sawant, S. V., Kiran, K., Singh, P. K., & Tuli, R. (2001). Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants. Plant Physiology, 126(4), 1630â1636.

Authors 4

Samir V. Sawant (first)
Kanti Kiran (additional)
Pradhyumna K. Singh (additional)
Rakesh Tuli (additional)

References 30 Referenced 62

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10.1002/j.1460-2075.1994.tb06668.x / EMBO J / D Positions +5 and +6 can be major determinants of the efficiency of non-AUG initiation for protein synthesis. by Boeck (1994)
10.1093/nar/15.4.1353 / Nucleic Acids Res / Comparison of the consensus sequence flanking translational start site in Drosophila and vertebrates. by Cavener (1987)
{'key': '2021062512142077600_R4', 'first-page': '679', 'article-title': 'Control of start codon choice on a plant viral RNA encoding overlapping genes.', 'volume': '5', 'author': 'Dinesh-Kumar', 'year': '1993', 'journal-title': 'Plant Cell'} / Plant Cell / Control of start codon choice on a plant viral RNA encoding overlapping genes. by Dinesh-Kumar (1993)
10.1002/j.1460-2075.1994.tb06669.x / EMBO J / The immediate downstream codon strongly influences the efficiency of utilization of eukaryotic translation initiation codons. by Grunert (1994)
10.1073/pnas.86.21.8247 / Proc Natl Acad Sci USA / Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side- chain length of the penultimate amino acid. by Hirel (1989)
10.1104/pp.119.2.713 / Plant Physiol / Use of ubiquitin fusions to augment protein expression in transgenic plants. by Hondred (1999)
{'key': '2021062512142077600_R8', 'first-page': '1', 'article-title': 'The GUS gene fusion system.', 'volume': 'B14', 'author': 'Jefferson', 'year': '1991', 'journal-title': 'Plant Molecular Biology Manual'} / Plant Molecular Biology Manual / The GUS gene fusion system. by Jefferson (1991)
10.1006/viro.1996.0370 / Virology / Both codon context and leader length contribute to efficient expression of two overlapping open reading frames of a cucumber necrosis virus bifunctional subgenomic mRNA. by Johnston (1996)
10.1023/A:1005816823636 / Plant Mol Biol / Context sequences of translation initiation codon in plants. by Joshi (1997)
10.1016/0022-2836(87)90418-9 / J Mol Biol / At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. by Kozak (1987)
10.1083/jcb.108.2.229 / J Cell Biol / The scanning model for translation: an update. by Kozak (1989)
10.1093/emboj/16.9.2482 / EMBO J / Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6. by Kozak (1997)
10.1046/j.1432-1327.1999.00024.x / Eur J Biochem / Analysis of a conditional degradation signal in yeast and mammalian cells. by Lèvy (1999)
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10.2144/00292bm09 / BioTechniques / Pretreatment of microprojecticles to improve the delivery of DNA in plant transformation. by Sawant (2000)
10.1007/BF00042344 / Plant Mol Biol / Post transcriptional regulation of nuclear-encoded genes in higher plants: the roles of mRNA stability and translation. by Sullivan (1993)
10.1007/BF00327214 / Mol Gen Genet / Optimizing the expression of chimeric genes in plant cells. by Taylor (1987)
10.1126/science.1962196 / Science / The N-end rule in bacteria. by Tobias (1991)
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Dates

Type	When
Created	22 years, 10 months ago (Oct. 1, 2002, 2:06 p.m.)
Deposited	4 years, 2 months ago (June 25, 2021, 8:15 a.m.)
Indexed	1 year, 3 months ago (May 14, 2024, 6:31 a.m.)
Issued	24 years ago (Aug. 1, 2001)
Published	24 years ago (Aug. 1, 2001)
Published Online	24 years ago (Aug. 1, 2001)
Published Print	24 years ago (Aug. 1, 2001)

Funders 0

None

BibTeX

@article{Sawant_2001, title={Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants}, volume={126}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.126.4.1630}, DOI={10.1104/pp.126.4.1630}, number={4}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Sawant, Samir V. and Kiran, Kanti and Singh, Pradhyumna K. and Tuli, Rakesh}, year={2001}, month=aug, pages={1630–1636} }

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          2001,
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      ]
    }
  },
  "URL": "http://dx.doi.org/10.1104/pp.126.4.1630",
  "relation": {},
  "ISSN": [
    "1532-2548",
    "0032-0889"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        2001,
        8,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        2001,
        8,
        1
      ]
    ]
  }
}