DOI: 10.1104/pp.125.2.770. The 5′ End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco

The 5′ End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco

10.1104/pp.125.2.770

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract Ferredoxin-1 (Fed-1) mRNA contains an internal light response element (iLRE) that destabilizes mRNA when light-grown plants are placed in darkness. mRNAs containing this element dissociate from polyribosomes in the leaves of transgenic tobacco (Nicotiana tabacum) plants transferred to the dark for 2 d. Here, we report in vivo labeling experiments with a chloramphenicol acetyl transferase mRNA fused to theFed-1 iLRE. Our data indicate that theFed-1 iLRE mediates a rapid decline in translational efficiency and that iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred to darkness. Both events occur before the decline in mRNA abundance, and polyribosome association is rapidly reversible if plants are re-illuminated. These observations support a model in which Fed-1 mRNA in illuminated leaves is stabilized by its association with polyribosomes, and/or by translation. In darkness a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded. We also show that a significant portion of total tobacco leaf mRNA is shifted from polyribosomal to non-polyribosomal fractions after 20 min in the dark, indicating that translation of other mRNAs is also rapidly down-regulated in response to darkness. This class includes some, but not all, cytoplasmic mRNAs encoding proteins involved in photosynthesis.

Bibliography

Hansen, E. R., Petracek, M. E., Dickey, L. F., & Thompson, W. F. (2001). The 5â² End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco. Plant Physiology, 125(2), 770â778.

Authors 4

Eric R. Hansen (first)
Marie E. Petracek (additional)
Lynn F. Dickey (additional)
William F. Thompson (additional)

References 24 Referenced 33

{'key': '2021062511264137300_R1', 'first-page': '10.16.11', 'article-title': 'Current protocols in molecular biology.', 'volume': '2', 'author': 'Ausubel', 'year': '1998', 'journal-title': 'Curr Protocols Mol Biol'} / Curr Protocols Mol Biol / Current protocols in molecular biology. by Ausubel (1998)
10.1007/BF00028908 / Plant Mol Biol / New plant binary vectors with selectable markers located proximal to the left T-DNA border. by Becker (1992)
10.1073/pnas.85.12.4190 / Proc Natl Acad Sci USA / mRNAs encoding ribulose-1,5-bisphosphate carboxylase remain bound to polysomes but are not translated in amaranth seedlings transferred to darkness. by Berry (1988)
10.1007/BF00019176 / Plant Mol Biol / Light-regulated expression of the Arabidopsis thaliana ferredoxin gene requires sequences upstream and downstream of the transcription initiation site. by Bovy (1995)
10.1111/j.1365-313X.1993.tb00019.x / Plant J / Promoter and leader regions involved in the expression of the Arabidopsis ferredoxin A gene. by Caspar (1993)
10.1016/S0091-679X(08)61032-8 / Methods Cell Biol / Methods for isolation and analysis of polyribosomes. by Davies (1995)
10.1002/j.1460-2075.1992.tb05290.x / EMBO J / Light regulatory sequences are located within the 5′ portion of the Fed-1 message sequence. by Dickey (1992)
{'key': '2021062511264137300_R8', 'first-page': '1171', 'article-title': 'Light modulation of ferredoxin mRNA abundance requires an open reading frame.', 'volume': '6', 'author': 'Dickey', 'year': '1994', 'journal-title': 'Plant Cell'} / Plant Cell / Light modulation of ferredoxin mRNA abundance requires an open reading frame. by Dickey (1994)
{'key': '2021062511264137300_R9', 'first-page': '475', 'article-title': 'Light regulation of Fed-1 mRNA requires an element in the 5′-untranslated region and correlates with differential polyribosome association.', 'volume': '10', 'author': 'Dickey', 'year': '1998', 'journal-title': 'Plant Cell'} / Plant Cell / Light regulation of Fed-1 mRNA requires an element in the 5′-untranslated region and correlates with differential polyribosome association. by Dickey (1998)
10.2307/3868959 / Plant Cell / cis-acting elements for light regulation of Pea Ferredoxin I gene expression are located within transcribed sequences. by Elliot (1989)
{'key': '2021062511264137300_R11', 'first-page': '389', 'article-title': 'Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings.', 'volume': '4', 'author': 'Gallo-Meagher', 'year': '1992', 'journal-title': 'Plant Cell'} / Plant Cell / Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings. by Gallo-Meagher (1992)
10.1016/S0091-679X(08)61047-X / Methods Cell Biol / Novel inducible/repressible gene expression. by Gatz (1995)
{'key': '2021062511264137300_R13', 'first-page': '545', 'article-title': 'Light-regulated modification and nuclear translocation of cytosolic G-Box binding factors in parsley.', 'volume': '6', 'author': 'Harter', 'year': '1994', 'journal-title': 'Plant Cell'} / Plant Cell / Light-regulated modification and nuclear translocation of cytosolic G-Box binding factors in parsley. by Harter (1994)
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10.1002/j.1460-2075.1995.tb07328.x / EMBO J / The stability of Escherichia coli lacZ mRNA depends upon simultaneity of its synthesis and translation. by Iost (1995)
10.1007/BF02667740 / Plant Mol Biol Rep / Assaying chimeric genes in plants: the GUS gene fusion system. by Jefferson (1987)
{'key': '2021062511264137300_R18', 'first-page': '174', 'article-title': 'Translational regulation in the chloroplast.', 'volume-title': 'A Look beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants.', 'author': 'Mayfield', 'year': '1998'} / A Look beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants. / Translational regulation in the chloroplast. by Mayfield (1998)
{'key': '2021062511264137300_R19', 'first-page': '96', 'article-title': 'Dependence of Fed-1 light regulation on translation.', 'volume-title': 'A Look beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants.', 'author': 'Petracek', 'year': '1998'} / A Look beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants. / Dependence of Fed-1 light regulation on translation. by Petracek (1998)
{'key': '2021062511264137300_R20', 'first-page': '2291', 'article-title': 'Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis.', 'volume': '9', 'author': 'Petracek', 'year': '1997', 'journal-title': 'Plant Cell'} / Plant Cell / Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis. by Petracek (1997)
10.1073/pnas.95.15.9009 / Proc Natl Acad Sci USA / Ferredoxin-1 mRNA is destabilized by changes in photosynthetic electron transport. by Petracek (1998)
10.1104/pp.93.2.572 / Plant Physiol / Isolation and characterization of a ferredoxin gene from Arabidopsis thaliana. by Somers (1990)
10.1146/annurev.pp.42.060191.002231 / Annu Rev Plant Physiol Plant Mol Biol / Physiological and molecular studies of light-regulated nuclear genes in higher plants. by Thompson (1991)
10.1007/BF00027495 / Plant Mol Biol / Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants. by Vorst (1990)

Dates

Type	When
Created	23 years ago (July 27, 2002, 12:59 a.m.)
Deposited	4 years, 1 month ago (June 25, 2021, 7:38 a.m.)
Indexed	1 year ago (Aug. 6, 2024, 6:36 a.m.)
Issued	24 years, 6 months ago (Feb. 1, 2001)
Published	24 years, 6 months ago (Feb. 1, 2001)
Published Online	24 years, 6 months ago (Feb. 1, 2001)
Published Print	24 years, 6 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Hansen_2001, title={The 5′ End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco}, volume={125}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.125.2.770}, DOI={10.1104/pp.125.2.770}, number={2}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Hansen, Eric R. and Petracek, Marie E. and Dickey, Lynn F. and Thompson, William F.}, year={2001}, month=feb, pages={770–778} }

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