DOI: 10.1104/pp.125.2.652. The Arabidopsis <i>ISR1</i> Locus Controlling Rhizobacteria-Mediated Induced Systemic Resistance Is Involved in Ethylene Signaling

The Arabidopsis ISR1 Locus Controlling Rhizobacteria-Mediated Induced Systemic Resistance Is Involved in Ethylene Signaling

10.1104/pp.125.2.652

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract In Arabidopsis, the rhizobacterial strain Pseudomonas fluorescens WCS417r triggers an induced systemic resistance (ISR) response that is effective against different types of pathogens. The ISR signaling pathway functions independent of salicylic acid, but requires responsiveness to jasmonate (JA) and ethylene. Using the genetic variability of ISR inducibility between Arabidopsis accessions, we recently identified a locus (ISR1) on chromosome III that is involved in ISR signaling. Accessions RLD and Wassilewskija (Ws) are recessive at the ISR1 locus and are, therefore, unable to develop ISR. Here we investigated whether theISR1 locus is involved in JA or ethylene signaling. Compared with the ISR-inducible accession Columbia (Col), accessions RLD and Ws were not affected in JA-induced inhibition of root growth and expression of the JA-responsive gene Atvsp, suggesting that the ISR1 locus is not involved in JA signaling. However, RLD and Ws showed an affected expression of the triple response and a reduced expression of the ethylene responsive genes Hel and Pdf1.2 after exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate. Moreover, in contrast to Col, RLD and Ws did not develop resistance against P. syringaepv. tomato DC3000 after treatment of the leaves with 1-aminocyclopropane-1-carboxylate. Analysis of the F2 and F3 progeny of a cross between Col (ISR1/ISR1) and RLD (isr1/isr1) revealed that reduced sensitivity to ethylene cosegregates with the recessive alleles of theISR1 locus. These results suggest that theISR1 locus encodes a component of the ethylene response, which is required for the expression of rhizobacteria-mediated ISR.

Bibliography

Ton, J., Davison, S., Van Wees, S. C. M., Van Loon, L. C., & Pieterse, C. M. J. (2001). The Arabidopsis ISR1 Locus Controlling Rhizobacteria-Mediated Induced Systemic Resistance Is Involved in Ethylene Signaling. Plant Physiology, 125(2), 652â661.

Authors 5

Jurriaan Ton (first)
Sylke Davison (additional)
Saskia C.M. Van Wees (additional)
L.C. Van Loon (additional)
Corné M.J. Pieterse (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 12:59 a.m.)
Deposited	4 years, 2 months ago (June 25, 2021, 7:37 a.m.)
Indexed	1 month, 1 week ago (July 30, 2025, 8:22 p.m.)
Issued	24 years, 7 months ago (Feb. 1, 2001)
Published	24 years, 7 months ago (Feb. 1, 2001)
Published Online	24 years, 7 months ago (Feb. 1, 2001)
Published Print	24 years, 7 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Ton_2001, title={The Arabidopsis ISR1 Locus Controlling Rhizobacteria-Mediated Induced Systemic Resistance Is Involved in Ethylene Signaling}, volume={125}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.125.2.652}, DOI={10.1104/pp.125.2.652}, number={2}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Ton, Jurriaan and Davison, Sylke and Van Wees, Saskia C.M. and Van Loon, L.C. and Pieterse, Corné M.J.}, year={2001}, month=feb, pages={652–661} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>In Arabidopsis, the rhizobacterial strain Pseudomonas fluorescens WCS417r triggers an induced systemic resistance (ISR) response that is effective against different types of pathogens. The ISR signaling pathway functions independent of salicylic acid, but requires responsiveness to jasmonate (JA) and ethylene. Using the genetic variability of ISR inducibility between Arabidopsis accessions, we recently identified a locus (ISR1) on chromosome III that is involved in ISR signaling. Accessions RLD and Wassilewskija (Ws) are recessive at the ISR1 locus and are, therefore, unable to develop ISR. Here we investigated whether theISR1 locus is involved in JA or ethylene signaling. Compared with the ISR-inducible accession Columbia (Col), accessions RLD and Ws were not affected in JA-induced inhibition of root growth and expression of the JA-responsive gene Atvsp, suggesting that the ISR1 locus is not involved in JA signaling. However, RLD and Ws showed an affected expression of the triple response and a reduced expression of the ethylene responsive genes Hel and Pdf1.2 after exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate. Moreover, in contrast to Col, RLD and Ws did not develop resistance against P. syringaepv. tomato DC3000 after treatment of the leaves with 1-aminocyclopropane-1-carboxylate. Analysis of the F2 and F3 progeny of a cross between Col (ISR1/ISR1) and RLD (isr1/isr1) revealed that reduced sensitivity to ethylene cosegregates with the recessive alleles of theISR1 locus. These results suggest that theISR1 locus encodes a component of the ethylene response, which is required for the expression of rhizobacteria-mediated ISR.</jats:p>",
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