DOI: 10.1104/pp.124.3.1305. Cloning and Characterization of a Receptor-Like Protein Kinase Gene Associated with Senescence

Cloning and Characterization of a Receptor-Like Protein Kinase Gene Associated with Senescence

10.1104/pp.124.3.1305

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract Senescence-associated genes are up-regulated during plant senescence and many have been implicated in encoding enzymes involved in the metabolism of senescing tissues. Using the differential display technique, we identified a SAG in bean (Phaseolus vulgaris) leaf that was exclusively expressed during senescence and was designated senescence-associated receptor-like kinase (SARK). The deduced SARK polypeptide consists of a signal peptide, a leucine-rich repeat in the extracellular region, a single membrane-spanning domain, and the characteristic serine/threonine protein kinase domain. The mRNA level for SARK increased prior to the loss of chlorophyll and the decrease of chlorophylla/b-binding protein mRNA. Detached mature bean leaves, which senesce at an accelerated rate compared with leaves on intact plants, showed a similar temporal pattern of SARK message accumulation. Light and cytokinin, which delayed the initiation of leaf senescence, also delayed SARK gene expression; in contrast, darkness and ethylene, which accelerated senescence, advanced the initial appearance of the SARK transcript. SARK protein accumulation exhibited a temporal pattern similar to that of its mRNA. A possible role for SARK in the regulation of leaf senescence was considered.

Bibliography

Hajouj, T., Michelis, R., & Gepstein, S. (2000). Cloning and Characterization of a Receptor-Like Protein Kinase Gene Associated with Senescence. Plant Physiology, 124(3), 1305â1314.

Authors 3

Taleb Hajouj (first)
Regina Michelis (additional)
Shimon Gepstein (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 1:01 a.m.)
Deposited	4 years, 2 months ago (June 25, 2021, 7:20 a.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:52 a.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Online	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Hajouj_2000, title={Cloning and Characterization of a Receptor-Like Protein Kinase Gene Associated with Senescence}, volume={124}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.124.3.1305}, DOI={10.1104/pp.124.3.1305}, number={3}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Hajouj, Taleb and Michelis, Regina and Gepstein, Shimon}, year={2000}, month=nov, pages={1305–1314} }

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