DOI: 10.1104/pp.122.1.127. Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga<i>Chlamydomonas reinhardtii</i>

Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green AlgaChlamydomonas reinhardtii

10.1104/pp.122.1.127

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract The work describes a novel approach for sustained photobiological production of H2 gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii. This single-organism, two-stage H2 production method circumvents the severe O2 sensitivity of the reversible hydrogenase by temporally separating photosynthetic O2 evolution and carbon accumulation (stage 1) from the consumption of cellular metabolites and concomitant H2 production (stage 2). A transition from stage 1 to stage 2 was effected upon S deprivation of the culture, which reversibly inactivated photosystem II (PSII) and O2 evolution. Under these conditions, oxidative respiration by the cells in the light depleted O2 and caused anaerobiosis in the culture, which was necessary and sufficient for the induction of the reversible hydrogenase. Subsequently, sustained cellular H2 gas production was observed in the light but not in the dark. The mechanism of H2 production entailed protein consumption and electron transport from endogenous substrate to the cytochromeb 6-f and PSI complexes in the chloroplast thylakoids. Light absorption by PSI was required for H2 evolution, suggesting that photoreduction of ferredoxin is followed by electron donation to the reversible hydrogenase. The latter catalyzes the reduction of protons to molecular H2in the chloroplast stroma.

Bibliography

Melis, A., Zhang, L., Forestier, M., Ghirardi, M. L., & Seibert, M. (2000). Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green AlgaChlamydomonas reinhardtii Â . Plant Physiology, 122(1), 127â136.

Authors 5

Anastasios Melis (first)
Liping Zhang (additional)
Marc Forestier (additional)
Maria L. Ghirardi (additional)
Michael Seibert (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 1:01 a.m.)
Deposited	4 years, 1 month ago (June 25, 2021, 5:49 a.m.)
Indexed	16 hours, 39 minutes ago (Aug. 21, 2025, 2:11 p.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	25 years, 7 months ago (Jan. 1, 2000)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Melis_2000, title={Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green AlgaChlamydomonas reinhardtii }, volume={122}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.122.1.127}, DOI={10.1104/pp.122.1.127}, number={1}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Melis, Anastasios and Zhang, Liping and Forestier, Marc and Ghirardi, Maria L. and Seibert, Michael}, year={2000}, month=jan, pages={127–136} }

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