DOI: 10.1104/pp.121.1.61. Arabidopsis Mutants Lacking the 43- and 54-Kilodalton Subunits of the Chloroplast Signal Recognition Particle Have Distinct Phenotypes

Arabidopsis Mutants Lacking the 43- and 54-Kilodalton Subunits of the Chloroplast Signal Recognition Particle Have Distinct Phenotypes

10.1104/pp.121.1.61

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

AbstractThe chloroplast signal recognition particle (cpSRP) is a protein complex consisting of 54- and 43-kD subunits encoded by the fifty-four chloroplast, which encodes cpSRP54 (ffc), and chaos (cao) loci, respectively. Two new null alleles in the ffc locus have been identified.ffc1-1 is caused by a stop codon in exon 10, whileffc1-2 has a large DNA insertion in intron 8.ffc mutants have yellow first true leaves that subsequently become green. The reaction center proteins D1, D2, and psaA/B, as well as seven different light-harvesting chlorophyll proteins (LHCPs), were found at reduced levels in the youngffc leaves but at wild-type levels in the older leaves. The abundance of the two types of LHCP was unaffected by the mutation, while two others were increased in the absence of cpSRP54. Null mutants in the cao locus contain reduced levels of the same subset of LHCP proteins as ffc mutants, but are distinguishable in four ways: young leaves are greener, the chlorophylla/b ratio is elevated, levels of reaction center proteins are normal, and there is no recovery in the level of LHCPs in the adult plant. The data suggest that cpSRP54 and cpSRP43 have some nonoverlapping roles and that alternative transport pathways can compensate for the absence of a functional cpSRP.

Bibliography

Amin, P., Sy, D. A. C., Pilgrim, M. L., Parry, D. H., Nussaume, L., & Hoffman, N. E. (1999). Arabidopsis Mutants Lacking the 43- and 54-Kilodalton Subunits of the Chloroplast Signal Recognition Particle Have Distinct Phenotypes. Plant Physiology, 121(1), 61â70.

Authors 6

Pinky Amin (first)
Donna A.C. Sy (additional)
Marsha L. Pilgrim (additional)
Devin H. Parry (additional)
Laurent Nussaume (additional)
Neil E. Hoffman (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 1:01 a.m.)
Deposited	1 year, 7 months ago (Jan. 5, 2024, 8:04 a.m.)
Indexed	2 weeks, 2 days ago (Aug. 5, 2025, 8:22 a.m.)
Issued	25 years, 11 months ago (Sept. 1, 1999)
Published	25 years, 11 months ago (Sept. 1, 1999)
Published Online	25 years, 11 months ago (Sept. 1, 1999)
Published Print	25 years, 11 months ago (Sept. 1, 1999)

Funders 0

None

BibTeX

@article{Amin_1999, title={Arabidopsis Mutants Lacking the 43- and 54-Kilodalton Subunits of the Chloroplast Signal Recognition Particle Have Distinct Phenotypes}, volume={121}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.121.1.61}, DOI={10.1104/pp.121.1.61}, number={1}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Amin, Pinky and Sy, Donna A.C. and Pilgrim, Marsha L. and Parry, Devin H. and Nussaume, Laurent and Hoffman, Neil E.}, year={1999}, month=sep, pages={61–70} }

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