DOI: 10.1104/pp.121.1.237. GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts

GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts

10.1104/pp.121.1.237

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract Protein import into chloroplasts is an energy-requiring process mediated by a proteinaceous import apparatus. Although previous work has shown that low levels of ATP or GTP can support precursor binding, the role of GTP during the import process remains unclear. Specifically, it is unknown whether GTP plays a separate role from ATP during the early stages of protein import and whether GTP has any role in the later stages of transport. We investigated the role of GTP during the various stages of protein import into chloroplasts by using purified GTP analogs and an in vitro import assay. GTP, GDP, the nonhydrolyzable analog GMP-PNP, and the slowly hydrolyzable analogs guanosine 5′-O-(2-thiodiphosphate) and guanosine 5′-O-(3-thiotriphosphate) were used in this study. Chromatographically purified 5′-guanylyl-imido-diphosphate and guanosine 5′-O-(3-thiotriphosphate) were found to inhibit the formation of early-import intermediates, even in the presence of ATP. We also observed that GTP does not play a role during the translocation of precursors from the intermediate state. We conclude that GTP hydrolysis influences events leading to the formation of early-import intermediates, but not subsequent steps such as precursor translocation.

Bibliography

Young, M. E., Keegstra, K., & Froehlich, J. E. (1999). GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts. Plant Physiology, 121(1), 237â244.

Authors 3

Michael E. Young (first)
Kenneth Keegstra (additional)
John E. Froehlich (additional)

References 38 Referenced 65

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 1:01 a.m.)
Deposited	4 years, 2 months ago (June 25, 2021, 5:34 a.m.)
Indexed	3 weeks ago (Aug. 7, 2025, 4:46 p.m.)
Issued	25 years, 11 months ago (Sept. 1, 1999)
Published	25 years, 11 months ago (Sept. 1, 1999)
Published Online	25 years, 11 months ago (Sept. 1, 1999)
Published Print	25 years, 11 months ago (Sept. 1, 1999)

Funders 0

None

BibTeX

@article{Young_1999, title={GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts}, volume={121}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.121.1.237}, DOI={10.1104/pp.121.1.237}, number={1}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Young, Michael E. and Keegstra, Kenneth and Froehlich, John E.}, year={1999}, month=sep, pages={237–244} }

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