DOI: 10.1104/pp.111.3.839. Biochemical Characterization of the Extracellular Phosphatases Produced by Phosphorus-Deprived Chlamydomonas reinhardtii

Biochemical Characterization of the Extracellular Phosphatases Produced by Phosphorus-Deprived Chlamydomonas reinhardtii

10.1104/pp.111.3.839

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract We have examined the extracellular phosphatases produced by the terrestrial green alga Chlamydomonas reinhardtii in response to phosphorus deprivation. Phosphorus-deprived cells increase extracellular alkaline phosphatase activity 300-fold relative to unstarved cells. The alkaline phosphatases are released into the medium by cell-wall-deficient strains and by wild-type cells after treatment with autolysin, indicating that they are localized to the periplasm. Anion-exchange chromatography and analysis by nondenaturing polyacrylamide gel electrophoresis revealed that there are two major inducible alkaline phosphatases. A calcium-dependent enzyme composed of 190-kD glycoprotein subunits accounts for 85 to 95% of the alkaline phosphatase activity. This phosphatase has optimal activity at pH 9.5 and a Km of 120 to 262 [mu]M for all physiological substrates tested, with the exception of phytic acid, which it cleaved with a 50-fold lower efficiency. An enzyme with optimal activity at pH 9 and no requirement for divalent cations accounts for 2 to 10% of the alkaline phosphatase activity. This phosphatase was only able to efficiently hydrolyze arylphosphates. The information reported here, in conjunction with the results of previous studies, defines the complement of extracellular phosphatases produced by phosphorus-deprived Chlamydomonas cells.

Authors 3

J. D. Quisel (first)
D. D. Wykoff (additional)
A. R. Grossman (additional)

References 0 Referenced 85

None

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 1:01 a.m.)
Deposited	4 years, 4 months ago (April 17, 2021, 3:21 a.m.)
Indexed	1 day, 9 hours ago (Aug. 29, 2025, 6:20 a.m.)
Issued	29 years, 1 month ago (July 1, 1996)
Published	29 years, 1 month ago (July 1, 1996)
Published Online	29 years, 1 month ago (July 1, 1996)
Published Print	29 years, 1 month ago (July 1, 1996)

Funders 0

None

BibTeX

@article{Quisel_1996, title={Biochemical Characterization of the Extracellular Phosphatases Produced by Phosphorus-Deprived Chlamydomonas reinhardtii}, volume={111}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.111.3.839}, DOI={10.1104/pp.111.3.839}, number={3}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={Quisel, J. D. and Wykoff, D. D. and Grossman, A. R.}, year={1996}, month=jul, pages={839–848} }

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