DOI: 10.1104/pp.103.032714. Profiling of Arabidopsis Secondary Metabolites by Capillary Liquid Chromatography Coupled to Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry

Profiling of Arabidopsis Secondary Metabolites by Capillary Liquid Chromatography Coupled to Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry

10.1104/pp.103.032714

Crossref journal-article

Oxford University Press (OUP)

Plant Physiology (286)

Abstract

Abstract Large-scale metabolic profiling is expected to develop into an integral part of functional genomics and systems biology. The metabolome of a cell or an organism is chemically highly complex. Therefore, comprehensive biochemical phenotyping requires a multitude of analytical techniques. Here, we describe a profiling approach that combines separation by capillary liquid chromatography with the high resolution, high sensitivity, and high mass accuracy of quadrupole time-of-flight mass spectrometry. About 2,000 different mass signals can be detected in extracts of Arabidopsis roots and leaves. Many of these originate from Arabidopsis secondary metabolites. Detection based on retention times and exact masses is robust and reproducible. The dynamic range is sufficient for the quantification of metabolites. Assessment of the reproducibility of the analysis showed that biological variability exceeds technical variability. Tools were optimized or established for the automatic data deconvolution and data processing. Subtle differences between samples can be detected as tested with the chalcone synthase deficient tt4 mutant. The accuracy of time-of-flight mass analysis allows to calculate elemental compositions and to tentatively identify metabolites. In-source fragmentation and tandem mass spectrometry can be used to gain structural information. This approach has the potential to significantly contribute to establishing the metabolome of Arabidopsis and other model systems. The principles of separation and mass analysis of this technique, together with its sensitivity and resolving power, greatly expand the range of metabolic profiling.

Bibliography

von Roepenack-Lahaye, E., Degenkolb, T., Zerjeski, M., Franz, M., Roth, U., Wessjohann, L., Schmidt, J., Scheel, D., & Clemens, S. (2004). Profiling of Arabidopsis Secondary Metabolites by Capillary Liquid Chromatography Coupled to Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry. Plant Physiology, 134(2), 548â559.

Authors 9

Edda von Roepenack-Lahaye (first)
Thomas Degenkolb (additional)
Michael Zerjeski (additional)
Mathias Franz (additional)
Udo Roth (additional)
Ludger Wessjohann (additional)
Jürgen Schmidt (additional)
Dierk Scheel (additional)
Stephan Clemens (additional)

References 31 Referenced 167

Dates

Type	When
Created	21 years, 6 months ago (Feb. 13, 2004, 3:43 p.m.)
Deposited	4 years, 2 months ago (June 27, 2021, 6:58 a.m.)
Indexed	3 months, 3 weeks ago (May 15, 2025, 5:03 p.m.)
Issued	21 years, 7 months ago (Feb. 1, 2004)
Published	21 years, 7 months ago (Feb. 1, 2004)
Published Online	21 years, 6 months ago (Feb. 13, 2004)
Published Print	21 years, 7 months ago (Feb. 1, 2004)

Funders 0

None

BibTeX

@article{von_Roepenack_Lahaye_2004, title={Profiling of Arabidopsis Secondary Metabolites by Capillary Liquid Chromatography Coupled to Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry}, volume={134}, ISSN={0032-0889}, url={http://dx.doi.org/10.1104/pp.103.032714}, DOI={10.1104/pp.103.032714}, number={2}, journal={Plant Physiology}, publisher={Oxford University Press (OUP)}, author={von Roepenack-Lahaye, Edda and Degenkolb, Thomas and Zerjeski, Michael and Franz, Mathias and Roth, Udo and Wessjohann, Ludger and Schmidt, Jürgen and Scheel, Dierk and Clemens, Stephan}, year={2004}, month=feb, pages={548–559} }

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  "ISSN": [
    "1532-2548",
    "0032-0889"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        2004,
        2,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        2004,
        2,
        1
      ]
    ]
  }
}