DOI: 10.1101/gr.7.5.422. An STS-Based Radiation Hybrid Map of the Human Genome

An STS-Based Radiation Hybrid Map of the Human Genome

10.1101/gr.7.5.422

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is <5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb.

Bibliography

Stewart, E. A., McKusick, K. B., Aggarwal, A., Bajorek, E., Brady, S., Chu, A., Fang, N., Hadley, D., Harris, M., Hussain, S., Lee, R., Maratukulam, A., OâConnor, K., Perkins, S., Piercy, M., Qin, F., Reif, T., Sanders, C., She, X., â¦ Cox, D. R. (1997). An STS-Based Radiation Hybrid Map of the Human Genome. Genome Research, 7(5), 422â433.

Authors 28

Elizabeth A. Stewart (first)
Kathleen B. McKusick (additional)
Amita Aggarwal (additional)
Ewa Bajorek (additional)
Shannon Brady (additional)
Angela Chu (additional)
Nicole Fang (additional)
David Hadley (additional)
Mark Harris (additional)
Sami Hussain (additional)
Richard Lee (additional)
Annu Maratukulam (additional)
Kyle O’Connor (additional)
Shanti Perkins (additional)
Mark Piercy (additional)
Fawn Qin (additional)
Tim Reif (additional)
Carla Sanders (additional)
Xiaohong She (additional)
Wei-Lin Sun (additional)
Poroshat Tabar (additional)
Susan Voyticky (additional)
Sid Cowles (additional)
Jian-Bing Fan (additional)
Chris Mader (additional)
John Quackenbush (additional)
Richard M. Myers (additional)
David R. Cox (additional)

References 33 Referenced 233

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Dates

Type	When
Created	8 years, 3 months ago (May 2, 2017, 8:25 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 1:33 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 7, 2025, 4:38 p.m.)
Issued	28 years, 4 months ago (May 1, 1997)
Published	28 years, 4 months ago (May 1, 1997)
Published Online	28 years, 4 months ago (May 1, 1997)
Published Print	28 years, 4 months ago (May 1, 1997)

Funders 0

None

BibTeX

@article{Stewart_1997, title={An STS-Based Radiation Hybrid Map of the Human Genome}, volume={7}, ISSN={1549-5469}, url={http://dx.doi.org/10.1101/gr.7.5.422}, DOI={10.1101/gr.7.5.422}, number={5}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Stewart, Elizabeth A. and McKusick, Kathleen B. and Aggarwal, Amita and Bajorek, Ewa and Brady, Shannon and Chu, Angela and Fang, Nicole and Hadley, David and Harris, Mark and Hussain, Sami and Lee, Richard and Maratukulam, Annu and O’Connor, Kyle and Perkins, Shanti and Piercy, Mark and Qin, Fawn and Reif, Tim and Sanders, Carla and She, Xiaohong and Sun, Wei-Lin and Tabar, Poroshat and Voyticky, Susan and Cowles, Sid and Fan, Jian-Bing and Mader, Chris and Quackenbush, John and Myers, Richard M. and Cox, David R.}, year={1997}, month=may, pages={422–433} }

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