DOI: 10.1101/gr.6.7.639. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.

A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.

10.1101/gr.6.7.639

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

Detecting and determining the relative abundance of diverse individual sequences in complex DNA samples is a recurring experimental challenge in analyzing genomes. We describe a general experimental approach to this problem, using microscopic arrays of DNA fragments on glass substrates for differential hybridization analysis of fluorescently labeled DNA samples. To test the system, 864 physically mapped lambda clones of yeast genomic DNA, together representing >75% of the yeast genome, were arranged into 1.8-cm x 1.8-cm arrays, each containing a total of 1744 elements. The microarrays were characterized by simultaneous hybridization of two different sets of isolated yeast chromosomes labeled with two different fluorophores. A laser fluorescent scanner was used to detect the hybridization signals from the two fluorophores. The results demonstrate the utility of DNA microarrays in the analysis of complex DNA samples. This system should find numerous applications in genome-wide genetic mapping, physical mapping, and gene expression studies.

Bibliography

Shalon, D., Smith, S. J., & Brown, P. O. (1996). A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Research, 6(7), 639â645.

Authors 3

D Shalon (first)
S J Smith (additional)
P O Brown (additional)

References 15 Referenced 796

10.1096/fasebj.5.1.1846833 / FASEB J. / New techniques for physical mapping of the human genome. (1991)
10.1016/0888-7543(92)90057-Y
10.1126/science.3538420
10.1126/science.8503011
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10.1126/science.1359641
10.1093/nar/20.7.1675
{'key': '2021111810255305000_6.7.639.9', 'first-page': '11', 'article-title': 'Genomic mismatch scanning: A new approach to genetic linkage mapping,', 'volume': '4', 'year': '1995', 'journal-title': 'Nature Genet.'} / Nature Genet. / Genomic mismatch scanning: A new approach to genetic linkage mapping, (1995)
10.1073/pnas.91.11.5022
10.1093/genetics/134.1.81 / Genetics / Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs. (1993)
Ross, M.T., J.D. Hoheisel, A.P. Monaco, Z. Larin, G. Zehetner, and H. Lehrach. 1992. High density gridded YAC filters: Their potential as genome mapping tools. In Techniques for the analysis of complex genomes (ed. Rakesh Anand), pp. 137–153. Academic Press, London, UK.
10.1126/science.270.5235.467
Shalon, D., 1995. “DNA micro arrays: A new tool for genetic analysis.” Ph.D. thesis, Stanford University, Stanford, CA.
10.1038/367489a0

Dates

Type	When
Created	18 years, 3 months ago (June 5, 2007, 5:18 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 1:28 p.m.)
Indexed	2 days, 16 hours ago (Sept. 3, 2025, 6 a.m.)
Issued	29 years, 2 months ago (July 1, 1996)
Published	29 years, 2 months ago (July 1, 1996)
Published Online	29 years, 2 months ago (July 1, 1996)
Published Print	29 years, 2 months ago (July 1, 1996)

Funders 0

None

BibTeX

@article{Shalon_1996, title={A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.}, volume={6}, ISSN={1088-9051}, url={http://dx.doi.org/10.1101/gr.6.7.639}, DOI={10.1101/gr.6.7.639}, number={7}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Shalon, D and Smith, S J and Brown, P O}, year={1996}, month=jul, pages={639–645} }

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