DOI: 10.1101/gr.362402. Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography

Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography

10.1101/gr.362402

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized toDrosophila melanogasterand mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.[The sequence data from this study have been submitted to GEO under accession nos. GPL208, GSM2409, GSM2410, GSM2411, GSM2412, GSM2413, GSM2414, GSE81, GSE82.]

Bibliography

Nuwaysir, E. F., Huang, W., Albert, T. J., Singh, J., Nuwaysir, K., Pitas, A., Richmond, T., Gorski, T., Berg, J. P., Ballin, J., McCormick, M., Norton, J., Pollock, T., Sumwalt, T., Butcher, L., Porter, D., Molla, M., Hall, C., Blattner, F., â¦ Green, R. D. (2002). Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography. Genome Research, 12(11), 1749â1755.

Authors 23

Emile F. Nuwaysir (first)
Wei Huang (additional)
Thomas J. Albert (additional)
Jaz Singh (additional)
Kate Nuwaysir (additional)
Alan Pitas (additional)
Todd Richmond (additional)
Tom Gorski (additional)
James P. Berg (additional)
Jeff Ballin (additional)
Mark McCormick (additional)
Jason Norton (additional)
Tim Pollock (additional)
Terry Sumwalt (additional)
Lawrence Butcher (additional)
DeAnn Porter (additional)
Michael Molla (additional)
Christine Hall (additional)
Fred Blattner (additional)
Michael R. Sussman (additional)
Rodney L. Wallace (additional)
Franco Cerrina (additional)
Roland D. Green (additional)

References 12 Referenced 292

10.1016/S0165-022X(99)00027-5
10.1126/science.1990438
10.1016/S0040-4020(97)00154-3
10.1038/86730
10.1021/ja964427a
10.1073/pnas.91.11.5022
Schena M. (1999) DNA microarrays: A practical approach. (Oxford University Press, Oxford, UK). (10.1093/oso/9780199637775.001.0001)
10.1126/science.270.5235.467
10.1093/nar/25.6.1155
{'key': '2021111810473260000_12.11.1749.10', 'first-page': '974', 'article-title': 'Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array Nat.', 'volume': '17', 'author': 'Singh-Gasson', 'year': '1999', 'journal-title': 'Biotech.'} / Biotech. / Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array Nat. by Singh-Gasson (1999)
10.1016/0888-7543(92)90014-J
10.1101/gad.901001

Dates

Type	When
Created	22 years, 9 months ago (Nov. 4, 2002, 6:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 7, 2024, 12:37 p.m.)
Indexed	2 months, 1 week ago (June 24, 2025, 7:28 a.m.)
Issued	22 years, 10 months ago (Nov. 1, 2002)
Published	22 years, 10 months ago (Nov. 1, 2002)
Published Online	22 years, 10 months ago (Nov. 1, 2002)
Published Print	22 years, 10 months ago (Nov. 1, 2002)

Funders 0

None

BibTeX

@article{Nuwaysir_2002, title={Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography}, volume={12}, ISSN={1549-5469}, url={http://dx.doi.org/10.1101/gr.362402}, DOI={10.1101/gr.362402}, number={11}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Nuwaysir, Emile F. and Huang, Wei and Albert, Thomas J. and Singh, Jaz and Nuwaysir, Kate and Pitas, Alan and Richmond, Todd and Gorski, Tom and Berg, James P. and Ballin, Jeff and McCormick, Mark and Norton, Jason and Pollock, Tim and Sumwalt, Terry and Butcher, Lawrence and Porter, DeAnn and Molla, Michael and Hall, Christine and Blattner, Fred and Sussman, Michael R. and Wallace, Rodney L. and Cerrina, Franco and Green, Roland D.}, year={2002}, month=nov, pages={1749–1755} }

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