DOI: 10.1101/gr.3155905. Disclosing hidden transcripts: Mouse natural sense–antisense transcripts tend to be poly(A) negative and nuclear localized

Disclosing hidden transcripts: Mouse natural sense–antisense transcripts tend to be poly(A) negative and nuclear localized

10.1101/gr.3155905

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

Genome-wide in silico analysis identified thousands of natural sense–antisense transcript (SAT) pairs in the mouse transcriptome. We investigated their expression using strand-specific oligo-microarray that distinguishes expression of sense and antisense RNA from 1947 SAT pairs. The majority of the predicted SATs are expressed at various steady-state levels in various tissues, and cluster analysis of the array data demonstrated that the ratio of sense and antisense expression for some of the SATs fluctuated markedly among these tissues, while the rest was unchanged. Surprisingly, further analyses indicated that vast amounts of multiple-sized transcripts are expressed from the SAT loci, which tended to be poly(A) negative, and nuclear localized. The tendency that the SATs are often not polyadenylated is conserved, even in the randomly chosen SAT genes in the plant Arabidopsis thaliana. Such common characteristics imply general roles of the SATs in regulation of gene expression.

Bibliography

Kiyosawa, H., Mise, N., Iwase, S., Hayashizaki, Y., & Abe, K. (2005). Disclosing hidden transcripts: Mouse natural senseâantisense transcripts tend to be poly(A) negative and nuclear localized. Genome Research, 15(4), 463â474.

Authors 5

Hidenori Kiyosawa (first)
Nathan Mise (additional)
Shigeru Iwase (additional)
Yoshihide Hayashizaki (additional)
Kuniya Abe (additional)

References 29 Referenced 98

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Dates

Type	When
Created	20 years, 5 months ago (March 21, 2005, 8:25 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 2:37 p.m.)
Indexed	3 months, 3 weeks ago (May 8, 2025, 3:25 p.m.)
Issued	20 years, 5 months ago (March 21, 2005)
Published	20 years, 5 months ago (March 21, 2005)
Published Online	20 years, 5 months ago (March 21, 2005)
Published Print	20 years, 5 months ago (April 1, 2005)

Funders 0

None

BibTeX

@article{Kiyosawa_2005, title={Disclosing hidden transcripts: Mouse natural sense–antisense transcripts tend to be poly(A) negative and nuclear localized}, volume={15}, ISSN={1088-9051}, url={http://dx.doi.org/10.1101/gr.3155905}, DOI={10.1101/gr.3155905}, number={4}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Kiyosawa, Hidenori and Mise, Nathan and Iwase, Shigeru and Hayashizaki, Yoshihide and Abe, Kuniya}, year={2005}, month=mar, pages={463–474} }

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