DOI: 10.1101/gr.182901. Surveying <i>Saccharomyces</i> Genomes to Identify Functional Elements by Comparative DNA Sequence Analysis

Surveying Saccharomyces Genomes to Identify Functional Elements by Comparative DNA Sequence Analysis

10.1101/gr.182901

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

Comparative sequence analysis has facilitated the discovery of protein coding genes and important functional sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of sequences of relatively closely related species. We tested the use of comparative DNA sequence analysis to aid identification of promoter regulatory elements, nonprotein-coding RNA genes, and small protein-coding genes by surveying random DNA sequences of severalSaccharomyces yeast species, with the goal of learning which species are best suited for comparisons with S. cerevisiae. We also determined the DNA sequence of a few specific promoters and RNA genes of several Saccharomyces species to determine the degree of conservation of known functional elements within the genome. Our results lead us to conclude that comparative DNA sequence analysis will enable identification of functionally conserved elements within the yeast genome, and suggest a path for obtaining this information.

Bibliography

Cliften, P. F., Hillier, L. W., Fulton, L., Graves, T., Miner, T., Gish, W. R., Waterston, R. H., & Johnston, M. (2001). Surveying Saccharomyces Genomes to Identify Functional Elements by Comparative DNA Sequence Analysis. Genome Research, 11(7), 1175â1186.

Authors 8

Paul F. Cliften (first)
LaDeana W. Hillier (additional)
Lucinda Fulton (additional)
Tina Graves (additional)
Tracie Miner (additional)
Warren R. Gish (additional)
Robert H. Waterston (additional)
Mark Johnston (additional)

References 45 Referenced 194

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{'key': '2021111810454931000_11.7.1175.15', 'first-page': '4999', 'article-title': 'Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae.', 'volume': '13', 'author': 'Griggs', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae. by Griggs (1993)
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{'key': '2021111810454931000_11.7.1175.45', 'first-page': '301', 'article-title': 'Flourescence-based DNA sequencing.', 'volume': 'I', 'author': 'Wilson', 'year': '1997', 'journal-title': 'Genome analysis: A laboratory manual'} / Genome analysis: A laboratory manual / Flourescence-based DNA sequencing. by Wilson (1997)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 2:03 p.m.)
Indexed	3 months ago (May 27, 2025, 5:06 p.m.)
Issued	24 years, 1 month ago (July 1, 2001)
Published	24 years, 1 month ago (July 1, 2001)
Published Online	24 years, 1 month ago (July 1, 2001)
Published Print	24 years, 1 month ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Cliften_2001, title={Surveying Saccharomyces Genomes to Identify Functional Elements by Comparative DNA Sequence Analysis}, volume={11}, ISSN={1549-5469}, url={http://dx.doi.org/10.1101/gr.182901}, DOI={10.1101/gr.182901}, number={7}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Cliften, Paul F. and Hillier, LaDeana W. and Fulton, Lucinda and Graves, Tina and Miner, Tracie and Gish, Warren R. and Waterston, Robert H. and Johnston, Mark}, year={2001}, month=jul, pages={1175–1186} }

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