DOI: 10.1101/gr.140200. Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells

Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells

10.1101/gr.140200

Crossref journal-article

Cold Spring Harbor Laboratory

Genome Research (246)

Abstract

Three hundred cDNAs containing putatively entire open reading frames (ORFs) for previously undefined genes were obtained from CD34+ hematopoietic stem/progenitor cells (HSPCs), based on EST cataloging, clone sequencing, in silico cloning, and rapid amplification of cDNA ends (RACE). The cDNA sizes ranged from 360 to 3496 bp and their ORFs coded for peptides of 58–752 amino acids. Public database search indicated that 225 cDNAs exhibited sequence similarities to genes identified across a variety of species. Homology analysis led to the recognition of 50 basic structural motifs/domains among these cDNAs. Genomic exon–intron organization could be established in 243 genes by integration of cDNA data with genome sequence information. Interestingly, a new gene named as HSPC070 on 3p was found to share a sequence of 105bp in 3′ UTR with RAF gene in reversed transcription orientation. Chromosomal localizations were obtained using electronic mapping for 192 genes and with radiation hybrid (RH) for 38 genes. Macroarray technique was applied to screen the gene expression patterns in five hematopoietic cell lines (NB4, HL60, U937, K562, and Jurkat) and a number of genes with differential expression were found. The resource work has provided a wide range of information useful not only for expression genomics and annotation of genomic DNA sequence, but also for further research on the function of genes involved in hematopoietic development and differentiation.[The sequence data described in this paper have been submitted to the GenBank data library under the accession nos. listed in Table 1, pp 1548–1552.]

Bibliography

Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., Han, Z.-G., Zhang, J.-W., Tao, J., Huang, Q.-H., Zhou, J., Hu, G.-X., Gu, J., â¦ Chen, Z. (2000). Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells. Genome Research, 10(10), 1546â1560.

Authors 21

Qing-Hua Zhang (first)
Min Ye (additional)
Xin-Yan Wu (additional)
Shuang-Xi Ren (additional)
Meng Zhao (additional)
Chun-Jun Zhao (additional)
Gang Fu (additional)
Yu Shen (additional)
Hui-Yong Fan (additional)
Gang Lu (additional)
Ming Zhong (additional)
Xiang-Ru Xu (additional)
Ze-Guang Han (additional)
Ji-Wang Zhang (additional)
Jiong Tao (additional)
Qiu-Hua Huang (additional)
Jun Zhou (additional)
Geng-Xi Hu (additional)
Jian Gu (additional)
Sai-Juan Chen (additional)
Zhu Chen (additional)

References 30 Referenced 144

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{'key': '2021111810350425000_10.10.1546.30', 'first-page': '302', 'article-title': 'Effect of retinoic acid isomers on proliferation, differentiation and PML relocalization in the APL cell line NB4.', 'volume': '9', 'author': 'Zhu', 'year': '1995', 'journal-title': 'Leukemia'} / Leukemia / Effect of retinoic acid isomers on proliferation, differentiation and PML relocalization in the APL cell line NB4. by Zhu (1995)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 18, 2021, 1:52 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 12, 2025, 5:50 p.m.)
Issued	24 years, 10 months ago (Oct. 1, 2000)
Published	24 years, 10 months ago (Oct. 1, 2000)
Published Online	24 years, 10 months ago (Oct. 1, 2000)
Published Print	24 years, 10 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Zhang_2000, title={Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells}, volume={10}, ISSN={1549-5469}, url={http://dx.doi.org/10.1101/gr.140200}, DOI={10.1101/gr.140200}, number={10}, journal={Genome Research}, publisher={Cold Spring Harbor Laboratory}, author={Zhang, Qing-Hua and Ye, Min and Wu, Xin-Yan and Ren, Shuang-Xi and Zhao, Meng and Zhao, Chun-Jun and Fu, Gang and Shen, Yu and Fan, Hui-Yong and Lu, Gang and Zhong, Ming and Xu, Xiang-Ru and Han, Ze-Guang and Zhang, Ji-Wang and Tao, Jiong and Huang, Qiu-Hua and Zhou, Jun and Hu, Geng-Xi and Gu, Jian and Chen, Sai-Juan and Chen, Zhu}, year={2000}, month=oct, pages={1546–1560} }

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