DOI: 10.1101/gad.989102. Characterization of HCP-6, a <i>C. elegans</i> protein required to prevent chromosome twisting and merotelic attachment

Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment

10.1101/gad.989102

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Previous studies of mitosis show that capture of single kinetochores by microtubules from both centrosomes (merotelic orientation) is a major cause of aneuploidy. We have characterized hcp-6, a temperature-sensitive chromosome segregation mutant in C. elegans that exhibits chromosomes attached to both poles via a single sister kinetochore. We demonstrate that the primary defect in this mutant is a failure to fully condense chromosomes during prophase. Although centromere formation and sister centromere resolution remain unaffected in hcp-6, the chromosomes lack the rigidity of wild-type chromosomes and twist around the long axis of the chromosome. As such, they are unable to establish a proper orientation at prometaphase, allowing individual kinetochores to be captured by microtubules from both poles. We therefore propose that chromosome rigidity plays an essential role in maintaining chromosome orientation to prevent merotelic capture.

Bibliography

Stear, J. H., & Roth, M. B. (2002). Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment. Genes & Development, 16(12), 1498â1508.

Authors 2

Jeffrey H. Stear (first)
Mark B. Roth (additional)

References 39 Referenced 78

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Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:41 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 11:08 a.m.)
Indexed	2 weeks, 3 days ago (Aug. 21, 2025, 1:17 p.m.)
Issued	23 years, 2 months ago (June 15, 2002)
Published	23 years, 2 months ago (June 15, 2002)
Published Online	23 years, 2 months ago (June 15, 2002)
Published Print	23 years, 2 months ago (June 15, 2002)

Funders 0

None

BibTeX

@article{Stear_2002, title={Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment}, volume={16}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.989102}, DOI={10.1101/gad.989102}, number={12}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Stear, Jeffrey H. and Roth, Mark B.}, year={2002}, month=jun, pages={1498–1508} }

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