DOI: 10.1101/gad.988802. Rap1–Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast

Rap1–Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast

10.1101/gad.988802

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

In Saccharomyces cerevisiae, heterochromatin-like regions are found near telomeres and at the silent mating-type loci, where they can repress genes in an epigenetic manner. Several proteins are involved in telomeric heterochromatin structure including Rap1, Sir2, Sir3, Sir4, yKu70 (Hdf1), yKu80 (Hdf2), and the N termini of histones H3 and H4. By recognizing cis-acting DNA-binding sites, Rap1 is believed to recruit Sir and other silencing proteins and determine where heterochromatin forms. The integrity of heterochromatin also requires the binding of Sir proteins to histones that may form a scaffold for Sir protein interactions with chromatin. In this study we describe how the heterochromatin complex may form initially and how it differs from the complex that spreads along the chromosome. We found that close to the telomere end, Sir4 can bind Rap1 independently of Sir2, Sir3, yKu70/yKu80, and the intact H4 N terminus. In contrast, Sir4 binding requires all of the silencing factors further along telomeric heterochromatin. These data indicate that Sir4 binding to Rap1 initiates the sequential association of Sir and other proteins, allowing the subsequent spreading of the heterochromatin proteins along the chromosome.

Bibliography

Luo, K., Vega-Palas, M. A., & Grunstein, M. (2002). Rap1âSir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast. Genes & Development, 16(12), 1528â1539.

Authors 3

Kunheng Luo (first)
Miguel A. Vega-Palas (additional)
Michael Grunstein (additional)

References 44 Referenced 184

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10.1093/genetics/143.1.81 / Genetics / Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae. by Liu (1996)
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{'key': '2021111508072378000_16.12.1528.26', 'first-page': '4441', 'article-title': 'Functional domains of Sir4, a gene required for position effect regulation in Saccharomyces cerevisiae.', 'volume': '7', 'author': 'Marshall', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Functional domains of Sir4, a gene required for position effect regulation in Saccharomyces cerevisiae. by Marshall (1987)
10.1016/S0092-8674(00)80773-4
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10.1101/gad.8.19.2257
10.1101/gad.7.7a.1133
10.1073/pnas.97.12.6658
10.1101/gad.8.12.1411
10.1101/gad.11.1.83
10.1101/sqb.1998.63.391
10.1016/S0092-8674(00)81671-2
10.1038/369245a0
10.1038/381251a0
10.1038/42288
10.1002/yea.320101310
10.1016/S0092-8674(00)81159-9
10.1101/gad.6.2.197

Dates

Type	When
Created	23 years ago (July 28, 2002, 6:41 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 11:08 a.m.)
Indexed	1 week, 2 days ago (Aug. 19, 2025, 7:03 a.m.)
Issued	23 years, 2 months ago (June 15, 2002)
Published	23 years, 2 months ago (June 15, 2002)
Published Online	23 years, 2 months ago (June 15, 2002)
Published Print	23 years, 2 months ago (June 15, 2002)

Funders 0

None

BibTeX

@article{Luo_2002, title={Rap1–Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast}, volume={16}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.988802}, DOI={10.1101/gad.988802}, number={12}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Luo, Kunheng and Vega-Palas, Miguel A. and Grunstein, Michael}, year={2002}, month=jun, pages={1528–1539} }

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