DOI: 10.1101/gad.972702. A molecular link between gene-specific and chromosome-wide transcriptional repression

A molecular link between gene-specific and chromosome-wide transcriptional repression

10.1101/gad.972702

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Gene-specific and chromosome-wide mechanisms of transcriptional regulation control development in multicellular organisms. SDC-2, the determinant of hermaphrodite fate in Caenorhabditis elegans, is a paradigm for both modes of regulation. SDC-2 represses transcription of X chromosomes to achieve dosage compensation, and it also represses the male sex-determination gene her-1 to elicit hermaphrodite differentiation. We show here that SDC-2 recruits the entire dosage compensation complex to her-1, directing thisX-chromosome repression machinery to silence an individual, autosomal gene. Functional dissection of her-1 in vivo revealed DNA recognition elements required for SDC-2 binding, recruitment of the dosage compensation complex, and transcriptional repression. Elements within her-1 differed in location, sequence, and strength of repression, implying that the dosage compensation complex may regulate transcription along the X chromosome using diverse recognition elements that play distinct roles in repression.

Bibliography

Chu, D. S., Dawes, H. E., Lieb, J. D., Chan, R. C., Kuo, A. F., & Meyer, B. J. (2002). A molecular link between gene-specific and chromosome-wide transcriptional repression. Genes & Development, 16(7), 796â805.

Authors 6

Diana S. Chu (first)
Heather E. Dawes (additional)
Jason D. Lieb (additional)
Raymond C. Chan (additional)
Annie F. Kuo (additional)
Barbara J. Meyer (additional)

References 31 Referenced 64

10.1016/0092-8674(91)90049-5
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10.1126/science.284.5421.1800
10.1093/genetics/133.4.875 / Genetics / Feedback control of sex determination by dosage compensation revealed through Caenorhabditis elegans sdc-3 mutations. by DeLong (1993)
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10.1128/MCB.21.5.1581-1592.2001

Dates

Type	When
Created	23 years ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 11:04 a.m.)
Indexed	6 days, 23 hours ago (Aug. 19, 2025, 6:17 a.m.)
Issued	23 years, 4 months ago (April 1, 2002)
Published	23 years, 4 months ago (April 1, 2002)
Published Online	23 years, 4 months ago (April 1, 2002)
Published Print	23 years, 4 months ago (April 1, 2002)

Funders 0

None

BibTeX

@article{Chu_2002, title={A molecular link between gene-specific and chromosome-wide transcriptional repression}, volume={16}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.972702}, DOI={10.1101/gad.972702}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Chu, Diana S. and Dawes, Heather E. and Lieb, Jason D. and Chan, Raymond C. and Kuo, Annie F. and Meyer, Barbara J.}, year={2002}, month=apr, pages={796–805} }

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  "abstract": "<jats:p>Gene-specific and chromosome-wide mechanisms of transcriptional regulation control development in multicellular organisms. SDC-2, the determinant of hermaphrodite fate in <jats:italic>Caenorhabditis elegans</jats:italic>, is a paradigm for both modes of regulation. SDC-2 represses transcription of <jats:italic>X</jats:italic> chromosomes to achieve dosage compensation, and it also represses the male sex-determination gene <jats:italic>her-1</jats:italic> to elicit hermaphrodite differentiation. We show here that SDC-2 recruits the entire dosage compensation complex to <jats:italic>her-1</jats:italic>, directing this<jats:italic>X</jats:italic>-chromosome repression machinery to silence an individual, autosomal gene. Functional dissection of <jats:italic>her-1</jats:italic> in vivo revealed DNA recognition elements required for SDC-2 binding, recruitment of the dosage compensation complex, and transcriptional repression. Elements within <jats:italic>her-1</jats:italic> differed in location, sequence, and strength of repression, implying that the dosage compensation complex may regulate transcription along the <jats:italic>X</jats:italic> chromosome using diverse recognition elements that play distinct roles in repression.</jats:p>",
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