DOI: 10.1101/gad.949802. E2F integrates cell cycle progression with DNA repair, replication, and G<sub>2</sub>/M checkpoints

E2F integrates cell cycle progression with DNA repair, replication, and G2/M checkpoints

10.1101/gad.949802

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The E2F transcription factor family is known to play a key role in the timely expression of genes required for cell cycle progression and proliferation, but only a few E2F target genes have been identified. We explored the possibility that E2F regulators play a broader role by identifying additional genes bound by E2F in living human cells. A protocol was developed to identify genomic binding sites for DNA-binding factors in mammalian cells that combines immunoprecipitation of cross-linked protein–DNA complexes with DNA microarray analysis. Among ∼1200 genes expressed during cell cycle entry, we found that the promoters of 127 were bound by the E2F4 transcription factor in primary fibroblasts. A subset of these targets was also bound by E2F1. Most previously identified target genes known to have roles in DNA replication and cell cycle control and represented on the microarray were confirmed by this analysis. We also identified a remarkable cadre of genes with no previous connection to E2F regulation, including genes that encode components of the DNA damage checkpoint and repair pathways, as well as factors involved in chromatin assembly/condensation, chromosome segregation, and the mitotic spindle checkpoint. Our data indicate that E2F directly links cell cycle progression with the coordinate regulation of genes essential for both the synthesis of DNA as well as its surveillance.

Bibliography

Ren, B., Cam, H., Takahashi, Y., Volkert, T., Terragni, J., Young, R. A., & Dynlacht, B. D. (2002). E2F integrates cell cycle progression with DNA repair, replication, and G2/M checkpoints. Genes & Development, 16(2), 245â256.

Authors 7

Bing Ren (first)
Hieu Cam (additional)
Yasuhiko Takahashi (additional)
Thomas Volkert (additional)
Jolyon Terragni (additional)
Richard A. Young (additional)
Brian David Dynlacht (additional)

References 38 Referenced 921

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:59 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:57 a.m.)
Indexed	14 minutes ago (Aug. 26, 2025, 11:32 p.m.)
Issued	23 years, 7 months ago (Jan. 15, 2002)
Published	23 years, 7 months ago (Jan. 15, 2002)
Published Online	23 years, 7 months ago (Jan. 15, 2002)
Published Print	23 years, 7 months ago (Jan. 15, 2002)

Funders 0

None

BibTeX

@article{Ren_2002, title={E2F integrates cell cycle progression with DNA repair, replication, and G2/M checkpoints}, volume={16}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.949802}, DOI={10.1101/gad.949802}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Ren, Bing and Cam, Hieu and Takahashi, Yasuhiko and Volkert, Thomas and Terragni, Jolyon and Young, Richard A. and Dynlacht, Brian David}, year={2002}, month=jan, pages={245–256} }

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