DOI: 10.1101/gad.945701. Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins

Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins

10.1101/gad.945701

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The anaphase-promoting complex/cyclosome (APC) ubiquitin ligase is activated by Cdc20 and Cdh1 and inhibited by Mad2 and the spindle assembly checkpoint complex, Mad2B, and the early mitotic inhibitor Emi1. Mad2 inhibits APCCdc20, whereas Mad2B preferentially inhibits APCCdh1. We have examined the mechanism of APC inhibition by Emi1 and find that unlike Mad2 proteins, Emi1 binds and inhibits both APCCdh1 and APCCdc20. Also unlike Mad2, Emi1 stabilizes cyclin A in the embryo and requires zinc for its APC inhibitory activity. We find that Emi1 binds the substrate-binding region of Cdc20 and prevents substrate binding to the APC, illustrating a novel mechanism of APC inhibition.

Authors 4

Julie D.R. Reimann (first)
Bryan E. Gardner (additional)
Florence Margottin-Goguet (additional)
Peter K. Jackson (additional)

References 44 Referenced 150

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:59 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:54 a.m.)
Indexed	3 days, 7 hours ago (Aug. 26, 2025, 3:13 a.m.)
Issued	23 years, 8 months ago (Dec. 15, 2001)
Published	23 years, 8 months ago (Dec. 15, 2001)
Published Online	23 years, 8 months ago (Dec. 15, 2001)
Published Print	23 years, 8 months ago (Dec. 15, 2001)

Funders 0

None

BibTeX

@article{Reimann_2001, title={Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.945701}, DOI={10.1101/gad.945701}, number={24}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Reimann, Julie D.R. and Gardner, Bryan E. and Margottin-Goguet, Florence and Jackson, Peter K.}, year={2001}, month=dec, pages={3278–3285} }

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  "abstract": "<jats:p>The anaphase-promoting complex/cyclosome (APC) ubiquitin ligase is activated by Cdc20 and Cdh1 and inhibited by Mad2 and the spindle assembly checkpoint complex, Mad2B, and the early mitotic inhibitor Emi1. Mad2 inhibits APC<jats:sup>Cdc20</jats:sup>, whereas Mad2B preferentially inhibits APC<jats:sup>Cdh1</jats:sup>. We have examined the mechanism of APC inhibition by Emi1 and find that unlike Mad2 proteins, Emi1 binds and inhibits both APC<jats:sup>Cdh1</jats:sup> and APC<jats:sup>Cdc20</jats:sup>. Also unlike Mad2, Emi1 stabilizes cyclin A in the embryo and requires zinc for its APC inhibitory activity. We find that Emi1 binds the substrate-binding region of Cdc20 and prevents substrate binding to the APC, illustrating a novel mechanism of APC inhibition.</jats:p>",
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