DOI: 10.1101/gad.935501. Mediator function of the human Rad51B–Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange

Mediator function of the human Rad51B–Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange

10.1101/gad.935501

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Five Rad51-like proteins, referred to as Rad51 paralogs, have been described in vertebrates. We show that two of them, Rad51B and Rad51C, are associated in a stable complex. Rad51B–Rad51C complex has ssDNA binding and ssDNA-stimulated ATPase activities. We also examined the functional interaction of Rad51B–Rad51C with Rad51 and RPA. Even though RPA enhances Rad51-catalyzed DNA joint formation via removal of secondary structure in the ssDNA substrate, it can also compete with Rad51 for binding to the substrate, leading to suppressed reaction efficiency. The competition by RPA for substrate binding can be partially alleviated by Rad51B–Rad51C. This recombination mediator function of Rad51B–Rad51C is likely required for the assembly of the Rad51-ssDNA nucleoprotein filament in vivo.

Bibliography

Sigurdsson, S., Van Komen, S., Bussen, W., Schild, D., Albala, J. S., & Sung, P. (2001). Mediator function of the human Rad51BâRad51C complex in Rad51/RPA-catalyzed DNA strand exchange. Genes & Development, 15(24), 3308â3318.

Authors 6

Stefan Sigurdsson (first)
Stephen Van Komen (additional)
Wendy Bussen (additional)
David Schild (additional)
Joanna S. Albala (additional)
Patrick Sung (additional)

References 33 Referenced 178

10.1093/emboj/16.17.5198
10.1006/jmbi.1999.2954
10.1016/S0968-0004(99)01451-6
10.1002/j.1460-2075.1994.tb06914.x / EMBO J. / Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA. by Benson (1994)
10.2741/A304 / Front. Biosci. / DNA strand exchange proteins: A biochemical and physical comparison. by Bianco (1998)
10.1074/jbc.M002075200
10.1038/sj.onc.1203283
10.1101/gad.12.14.2208
10.1073/pnas.121046198
10.1073/pnas.94.2.463
10.1016/S0021-9258(19)78100-9 / J. Biol. Chem. / Recombinant replication protein A: Expression, complex formation, and functional characterization. by Henricksen (1994)
10.1073/pnas.091603098
10.1073/pnas.111005698
10.1016/S1097-2765(00)80202-6
10.1016/S1097-2765(01)00174-5 / Mol. Cell / BRCA2 is required for homology-directed repair of chromosomal breaks. by Moynahan (2001)
10.1038/34950
10.1126/science.8456314
10.1128/MMBR.63.2.349-404.1999 / Microbiol. Mol. Biol. Rev. / Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. by Paques (1999)
10.1101/gad.826100
10.1016/S0079-6603(08)61005-3
10.1074/jbc.M001473200
10.1038/34943
10.1074/jbc.M010011200
10.1074/jbc.272.12.7940
10.1074/jbc.272.45.28194
10.1101/gad.11.9.1111
10.1016/0092-8674(95)90434-4
10.1073/pnas.84.24.8951
10.1016/S0027-5107(00)00054-3
10.1128/MCB.20.17.6476-6482.2000
10.1128/MCB.21.8.2858-2866.2001
10.1016/S0027-5107(01)00115-4
10.1073/pnas.90.9.3875

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:59 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:53 a.m.)
Indexed	1 week, 5 days ago (Aug. 19, 2025, 6:37 a.m.)
Issued	23 years, 8 months ago (Dec. 15, 2001)
Published	23 years, 8 months ago (Dec. 15, 2001)
Published Online	23 years, 8 months ago (Dec. 15, 2001)
Published Print	23 years, 8 months ago (Dec. 15, 2001)

Funders 0

None

BibTeX

@article{Sigurdsson_2001, title={Mediator function of the human Rad51B–Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.935501}, DOI={10.1101/gad.935501}, number={24}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Sigurdsson, Stefan and Van Komen, Stephen and Bussen, Wendy and Schild, David and Albala, Joanna S. and Sung, Patrick}, year={2001}, month=dec, pages={3308–3318} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        19
      ]
    ],
    "date-time": "2025-08-19T10:37:32Z",
    "timestamp": 1755599852426
  },
  "reference-count": 33,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "24",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Five Rad51-like proteins, referred to as Rad51 paralogs, have been described in vertebrates. We show that two of them, Rad51B and Rad51C, are associated in a stable complex. Rad51B\u2013Rad51C complex has ssDNA binding and ssDNA-stimulated ATPase activities. We also examined the functional interaction of Rad51B\u2013Rad51C with Rad51 and RPA. Even though RPA enhances Rad51-catalyzed DNA joint formation via removal of secondary structure in the ssDNA substrate, it can also compete with Rad51 for binding to the substrate, leading to suppressed reaction efficiency. The competition by RPA for substrate binding can be partially alleviated by Rad51B\u2013Rad51C. This recombination mediator function of Rad51B\u2013Rad51C is likely required for the assembly of the Rad51-ssDNA nucleoprotein filament in vivo.</jats:p>",
  "DOI": "10.1101/gad.935501",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T23:59:15Z",
    "timestamp": 1027727955000
  },
  "page": "3308-3318",
  "source": "Crossref",
  "is-referenced-by-count": 178,
  "title": "Mediator function of the human Rad51B\u2013Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange",
  "prefix": "10.1101",
  "volume": "15",
  "author": [
    {
      "given": "Stefan",
      "family": "Sigurdsson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Stephen",
      "family": "Van Komen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Wendy",
      "family": "Bussen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "David",
      "family": "Schild",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Joanna S.",
      "family": "Albala",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Patrick",
      "family": "Sung",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        2001,
        12,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111507525717000_15.24.3308.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.17.5198"
    },
    {
      "key": "2021111507525717000_15.24.3308.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1999.2954"
    },
    {
      "key": "2021111507525717000_15.24.3308.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(99)01451-6"
    },
    {
      "key": "2021111507525717000_15.24.3308.4",
      "doi-asserted-by": "crossref",
      "first-page": "5764",
      "DOI": "10.1002/j.1460-2075.1994.tb06914.x",
      "article-title": "Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.",
      "volume": "23",
      "author": "Benson",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111507525717000_15.24.3308.5",
      "doi-asserted-by": "crossref",
      "first-page": "D570",
      "DOI": "10.2741/A304",
      "article-title": "DNA strand exchange proteins: A biochemical and physical comparison.",
      "volume": "3",
      "author": "Bianco",
      "year": "1998",
      "journal-title": "Front. Biosci."
    },
    {
      "key": "2021111507525717000_15.24.3308.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M002075200"
    },
    {
      "key": "2021111507525717000_15.24.3308.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.onc.1203283"
    },
    {
      "key": "2021111507525717000_15.24.3308.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.14.2208"
    },
    {
      "key": "2021111507525717000_15.24.3308.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.121046198"
    },
    {
      "key": "2021111507525717000_15.24.3308.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.2.463"
    },
    {
      "key": "2021111507525717000_15.24.3308.11",
      "doi-asserted-by": "crossref",
      "first-page": "11121",
      "DOI": "10.1016/S0021-9258(19)78100-9",
      "article-title": "Recombinant replication protein A: Expression, complex formation, and functional characterization.",
      "volume": "269",
      "author": "Henricksen",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111507525717000_15.24.3308.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.091603098"
    },
    {
      "key": "2021111507525717000_15.24.3308.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.111005698"
    },
    {
      "key": "2021111507525717000_15.24.3308.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80202-6"
    },
    {
      "key": "2021111507525717000_15.24.3308.15",
      "doi-asserted-by": "crossref",
      "first-page": "263",
      "DOI": "10.1016/S1097-2765(01)00174-5",
      "article-title": "BRCA2 is required for homology-directed repair of chromosomal breaks.",
      "volume": "2",
      "author": "Moynahan",
      "year": "2001",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "2021111507525717000_15.24.3308.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/34950"
    },
    {
      "key": "2021111507525717000_15.24.3308.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8456314"
    },
    {
      "key": "2021111507525717000_15.24.3308.18",
      "doi-asserted-by": "crossref",
      "first-page": "349",
      "DOI": "10.1128/MMBR.63.2.349-404.1999",
      "article-title": "Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.",
      "volume": "63",
      "author": "Paques",
      "year": "1999",
      "journal-title": "Microbiol. Mol. Biol. Rev."
    },
    {
      "key": "2021111507525717000_15.24.3308.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.826100"
    },
    {
      "key": "2021111507525717000_15.24.3308.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6603(08)61005-3"
    },
    {
      "key": "2021111507525717000_15.24.3308.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M001473200"
    },
    {
      "key": "2021111507525717000_15.24.3308.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/34943"
    },
    {
      "key": "2021111507525717000_15.24.3308.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M010011200"
    },
    {
      "key": "2021111507525717000_15.24.3308.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.12.7940"
    },
    {
      "key": "2021111507525717000_15.24.3308.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.45.28194"
    },
    {
      "key": "2021111507525717000_15.24.3308.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.9.1111"
    },
    {
      "key": "2021111507525717000_15.24.3308.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90434-4"
    },
    {
      "key": "2021111507525717000_15.24.3308.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.24.8951"
    },
    {
      "key": "2021111507525717000_15.24.3308.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0027-5107(00)00054-3"
    },
    {
      "key": "2021111507525717000_15.24.3308.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.20.17.6476-6482.2000"
    },
    {
      "key": "2021111507525717000_15.24.3308.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.21.8.2858-2866.2001"
    },
    {
      "key": "2021111507525717000_15.24.3308.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0027-5107(01)00115-4"
    },
    {
      "key": "2021111507525717000_15.24.3308.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.9.3875"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.935501",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T15:53:22Z",
    "timestamp": 1636991602000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.935501"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        12,
        15
      ]
    ]
  },
  "references-count": 33,
  "journal-issue": {
    "issue": "24",
    "published-print": {
      "date-parts": [
        [
          2001,
          12,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.935501"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.935501",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2001,
        12,
        15
      ]
    ]
  }
}