DOI: 10.1101/gad.931401. Histone H3 specific acetyltransferases are essential for cell cycle progression

Histone H3 specific acetyltransferases are essential for cell cycle progression

10.1101/gad.931401

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Longstanding observations suggest that acetylation and/or amino-terminal tail structure of histones H3 and H4 are critical for eukaryotic cells. For Saccharomyces cerevisiae, loss of a single H4-specific histone acetyltransferase (HAT), Esa1p, results in cell cycle defects and death. In contrast, although several yeast HAT complexes preferentially acetylate histone H3, the catalytic subunits of these complexes are not essential for viability. To resolve the apparent paradox between the significance of H3 versus H4 acetylation, we tested the hypothesis that H3 modification is essential, but is accomplished through combined activities of two enzymes. We observed that Sas3p and Gcn5p HAT complexes have overlapping patterns of acetylation. Simultaneous disruption of SAS3, the homolog of the MOZ leukemia gene, and GCN5, the hGCN5/PCAFhomolog, is synthetically lethal due to loss of acetyltransferase activity. This key combination of activities is specific for these two HATs because neither is synthetically lethal with mutations of other MYST family or H3-specific acetyltransferases. Further, the combined loss of GCN5 and SAS3 functions results in an extensive, global loss of H3 acetylation and arrest in the G2/M phase of the cell cycle. The strikingly similar effect of loss of combined essential H3 HAT activities and the loss of a single essential H4 HAT underscores the fundamental biological significance of each of these chromatin-modifying activities.

Bibliography

Howe, L., Auston, D., Grant, P., John, S., Cook, R. G., Workman, J. L., & Pillus, L. (2001). Histone H3 specific acetyltransferases are essential for cell cycle progression. Genes & Development, 15(23), 3144â3154.

Authors 7

LeAnn Howe (first)
Darryl Auston (additional)
Patrick Grant (additional)
Sam John (additional)
Richard G. Cook (additional)
Jerry L. Workman (additional)
Lorraine Pillus (additional)

References 70 Referenced 187

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{'key': '2021111507523829000_15.23.3144.33', 'first-page': '4111', 'article-title': 'The highly conserved N-terminal domains of histones H3 and H4 are required for normal cell cycle progression.', 'volume': '11', 'author': 'Morgan', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The highly conserved N-terminal domains of histones H3 and H4 are required for normal cell cycle progression. by Morgan (1991)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 7:59 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:54 a.m.)
Indexed	3 weeks, 5 days ago (Aug. 6, 2025, 8:53 a.m.)
Issued	23 years, 9 months ago (Dec. 1, 2001)
Published	23 years, 9 months ago (Dec. 1, 2001)
Published Online	23 years, 9 months ago (Dec. 1, 2001)
Published Print	23 years, 9 months ago (Dec. 1, 2001)

Funders 0

None

BibTeX

@article{Howe_2001, title={Histone H3 specific acetyltransferases are essential for cell cycle progression}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.931401}, DOI={10.1101/gad.931401}, number={23}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Howe, LeAnn and Auston, Darryl and Grant, Patrick and John, Sam and Cook, Richard G. and Workman, Jerry L. and Pillus, Lorraine}, year={2001}, month=dec, pages={3144–3154} }

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