DOI: 10.1101/gad.9.6.675. Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors.

Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors.

10.1101/gad.9.6.675

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

TFIIIC2 is a general factor essential for transcription of 5S RNA, tRNA, and VA RNA genes by mammalian RNA polymerase III and consists of two forms designated TFIIIC2a and TFIIIC2b. TFIIIC2a and TFIIIC2b share common subunits of 220, 102, 90, and 63 kD but differ with respect to transcription activity and the presence of a presumptive 110-kD subunit in the active form (TFIIIC2a). Because both forms can bind the promoter directly, a selective role for the 110-kD subunit in the regulation of RNA polymerase III activity has been suggested. To investigate this possibility, we have cloned and expressed a cDNA encoding the 110-kD subunit (TFIIIC beta). Immunoprecipitation studies with anti-TFIIIC beta antibodies have confirmed that TFIIIC beta is a bona fide subunit present only in TFIIIC2a, that TFIIIC2a and the general factor TFIIIC1 are associated in unfractionated extracts, and that previously undetected polypeptides (potential TFIIIC1 subunits) can be isolated in association with TFIIIC2a. Previous studies have shown that increases in RNA polymerase III activity during infection of cells by adenovirus (with concomitant E1A expression) or during cell growth at high serum concentration results from an increased activity in the TFIIIC fraction. Studies with antibodies to TFIIIC beta have shown that this is strongly correlated with a selective increase in the cellular concentration of the TFIIIC beta 110-kD subunit and a concomitant rise in the ratio of the active-to-inactive forms of TFIIIC2.

Bibliography

Sinn, E., Wang, Z., Kovelman, R., & Roeder, R. G. (1995). Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors. Genes & Development, 9(6), 675â685.

Authors 4

E Sinn (first)
Z Wang (additional)
R Kovelman (additional)
R G Roeder (additional)

References 51 Referenced 52

10.1006/jmbi.1990.9999
10.1002/j.1460-2075.1990.tb07389.x / EMBO J. / The subunit structure of Saccharomyces cerevisiae transcription factor IIIC probed with a novel photocrosslinking reagent. (1990)
10.1146/annurev.ge.20.120186.000401
10.1093/nar/13.4.1413
Boguski, M.S., Sikorski, R.S. Hieter, P. and Goebl. M. 1990. Expanding family. Nature 346: 114. (10.1038/346114a0)
10.1101/gad.4.8.1345
10.1038/362475a0
10.1002/j.1460-2075.1993.tb05936.x / EMBO J. / Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III. (1993)
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10.1038/363176a0
{'key': '2021111418212878000_9.6.675.13', 'first-page': '3880', 'article-title': 'Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: Inactivation of specific transcription factors.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: Inactivation of specific transcription factors. (1987)
10.1016/0968-0004(91)90166-S
10.1016/0092-8674(83)90011-9
10.1126/science.2996135
Geiduschek, E.P. and G.A. Kassavetis. 1992. RNA polymerase III transcription complexes. In Transcription regulation (ed. K. Yamamoto), pp. 247–280. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.
10.1146/annurev.bi.57.070188.004301
10.1146/annurev.bi.56.070187.003151
10.1016/0968-0004(91)90070-C
Harlow, E. and D. Lane. 1988. Antibodies: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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{'key': '2021111418212878000_9.6.675.26', 'first-page': '503', 'article-title': 'The gene complementing a temperature-sensitive cell cycle mutant of BHK cells is the human homologue of the yeast RPC53 gene, which encodes a subunit of RNA polymerase C (III).', 'volume': '4', 'year': '1993', 'journal-title': 'Cell Growth Differ.'} / Cell Growth Differ. / The gene complementing a temperature-sensitive cell cycle mutant of BHK cells is the human homologue of the yeast RPC53 gene, which encodes a subunit of RNA polymerase C (III). (1993)
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{'key': '2021111418212878000_9.6.675.29', 'first-page': '7859', 'article-title': 'Molecular cloning, expression, and characterization of the Drosophila 85-kilodalton TFIID subunit.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Molecular cloning, expression, and characterization of the Drosophila 85-kilodalton TFIID subunit. (1993)
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{'key': '2021111418212878000_9.6.675.34', 'first-page': '3053', 'article-title': 'Cloning and characterization of an evolutionary-divergent DNA-binding subunit of mammalian TFIIIC.', 'volume': '14', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Cloning and characterization of an evolutionary-divergent DNA-binding subunit of mammalian TFIIIC. (1994)
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{'key': '2021111418212878000_9.6.675.39', 'first-page': '2894', 'article-title': 'Cloning of TFC1, the Saccharomyces cerevisiae gene encoding the 95-kDa subunit of transcription factor TFIIIC.', 'volume': '5', 'year': '1992', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Cloning of TFC1, the Saccharomyces cerevisiae gene encoding the 95-kDa subunit of transcription factor TFIIIC. (1992)
10.1016/S0021-9258(17)32324-4 / J. Biol. Chem. / Multiple sets of basal factors initiate transcription by RNA polymerase II. (1994)
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10.1016/S0021-9258(18)81682-9 / J. Biol. Chem. / Purification and characterization of transcription factor IIIC2. (1989)
10.1146/annurev.bi.60.070191.003353

Dates

Type	When
Created	18 years, 3 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:31 p.m.)
Indexed	1 year, 7 months ago (Jan. 25, 2024, 12:47 p.m.)
Issued	30 years, 5 months ago (March 15, 1995)
Published	30 years, 5 months ago (March 15, 1995)
Published Online	30 years, 5 months ago (March 15, 1995)
Published Print	30 years, 5 months ago (March 15, 1995)

Funders 0

None

BibTeX

@article{Sinn_1995, title={Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.6.675}, DOI={10.1101/gad.9.6.675}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Sinn, E and Wang, Z and Kovelman, R and Roeder, R G}, year={1995}, month=mar, pages={675–685} }

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