Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3.
10.1101/gad.9.21.2635
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Fibroblast growth factor 4 (FGF-4) has been shown to be a signaling molecule whose expression is essential for postimplantation mouse development and, at later embryonic stages, for limb patterning and growth. The FGF-4 gene is expressed in the blastocyst inner cell mass and later in distinct embryonic tissues but is transcriptionally silent in the adult. In tissue culture FGF-4 expression is restricted to undifferentiated embryonic stem (ES) cells and embryonal carcinoma (EC) cell lines. Previously, we determined that EC cell-specific transcriptional activation of the FGF-4 gene depends on a synergistic interaction between octamer-binding proteins and an EC-specific factor, Fx, that bind adjacent sites on the FGF-4 enhancer. Through the cloning and characterization of an F9 cell cDNA we now show that the latter activity is Sox2, a member of the Sry-related Sox factors family. Sox2 can form a ternary complex with either the ubiquitous Oct-1 or the embryonic-specific Oct-3 protein on FGF-4 enhancer DNA sequences. However, only the Sox2/Oct-3 complex is able to promote transcriptional activation. These findings identify FGF-4 as the first known embryonic target gene for Oct-3 and for any of the Sox factors, and offer insights into the mechanisms of selective gene activation by Sox and octamer-binding proteins during embryogenesis.

Bibliography

Yuan, H., Corbi, N., Basilico, C., & Dailey, L. (1995). Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3. Genes & Development, 9(21), 2635–2645.

Authors 4
  1. H Yuan (first)
  2. N Corbi (additional)
  3. C Basilico (additional)
  4. L Dailey (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:40 p.m.)
Indexed 2 weeks, 4 days ago (Aug. 5, 2025, 8:45 a.m.)
Issued 29 years, 9 months ago (Nov. 1, 1995)
Published 29 years, 9 months ago (Nov. 1, 1995)
Published Online 29 years, 9 months ago (Nov. 1, 1995)
Published Print 29 years, 9 months ago (Nov. 1, 1995)
Funders 0

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@article{Yuan_1995, title={Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.21.2635}, DOI={10.1101/gad.9.21.2635}, number={21}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Yuan, H and Corbi, N and Basilico, C and Dailey, L}, year={1995}, month=nov, pages={2635–2645} }