DOI: 10.1101/gad.9.20.2445. The HCF repeat is an unusual proteolytic cleavage signal.

The HCF repeat is an unusual proteolytic cleavage signal.

10.1101/gad.9.20.2445

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The herpes simplex virus VP16-associated protein HCF is a nuclear host-cell factor that exists as a family of polypeptides encoded by a single gene. The mature HCF polypeptides are amino- and carboxy-terminal fragments of a large approximately 300-kD precursor protein that arise through cleavage at one or more centrally located sites. The sites of cleavage are the HCF repeats, highly conserved 26-amino-acid sequences repeated six times in the HCF precursor protein. The HCF repeat alone is sufficient to induce cleavage of a heterologous protein, and cleavage occurs at a defined site--PPCE/THET--within the HCF repeat. Alanine-scan mutagenesis was used to identify a large 18-amino-acid segment of the HCF repeat that is important to induce cleavage of a heterologous protein. Even though HCF is cleaved, the majority of amino- and carboxy-terminal cleavage products remain tightly, albeit noncovalently, associated. Modulation of this noncovalent association may provide a mechanism for regulating HCF activity. For example, the cleaved products of an alternative mRNA splicing variant of HCF do not remain associated.

Bibliography

Wilson, A. C., Peterson, M. G., & Herr, W. (1995). The HCF repeat is an unusual proteolytic cleavage signal. Genes & Development, 9(20), 2445â2458.

Authors 3

A C Wilson (first)
M G Peterson (additional)
W Herr (additional)

References 39 Referenced 73

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10.1101/gad.7.11.2235

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:40 p.m.)
Indexed	3 weeks ago (Aug. 6, 2025, 9:26 a.m.)
Issued	29 years, 10 months ago (Oct. 15, 1995)
Published	29 years, 10 months ago (Oct. 15, 1995)
Published Online	29 years, 10 months ago (Oct. 15, 1995)
Published Print	29 years, 10 months ago (Oct. 15, 1995)

Funders 0

None

BibTeX

@article{Wilson_1995, title={The HCF repeat is an unusual proteolytic cleavage signal.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.20.2445}, DOI={10.1101/gad.9.20.2445}, number={20}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Wilson, A C and Peterson, M G and Herr, W}, year={1995}, month=oct, pages={2445–2458} }

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