DOI: 10.1101/gad.9.18.2214. Altering specific telomerase RNA template residues affects active site function.

Altering specific telomerase RNA template residues affects active site function.

10.1101/gad.9.18.2214

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying a template sequence in the telomerase RNA. We studied the functional roles of specific residues in the Tetrahymena telomerase RNA template region. Unexpectedly, mutation of certain templating residues caused dramatic effects on specific aspects of the enzyme reaction, including loss of enzymatic fidelity and premature product dissociation. None of these fundamental changes in enzymatic action are explainable by altered base-pairing between the telomerase RNA and DNA substrate. These influences of specific template bases of the telomerase RNA on enzymatic properties of telomerase provide evidence for critical roles of these RNA residues in two active site functions--fidelity and DNA substrate/enzyme interaction.

Bibliography

Gilley, D., Lee, M. S., & Blackburn, E. H. (1995). Altering specific telomerase RNA template residues affects active site function. Genes & Development, 9(18), 2214â2226.

Authors 3

D Gilley (first)
M S Lee (additional)
E H Blackburn (additional)

References 37 Referenced 74

10.1101/gad.8.5.563
Bebenek, K. and T.A. Kunkel. 1993. The fidelity of retroviral reverse transcriptases. In Reverse transcriptase. (ed. A.M. Skalka and S.P. Goff), pp. 85–103. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
10.1126/science.8469987
10.1073/pnas.87.5.1758
10.1002/j.1460-2075.1994.tb06910.x / EMBO J. / Architecture of telomerase RNA. (1994)
10.1126/science.8128220
10.1146/annurev.bi.61.070192.000553
10.1016/0092-8674(94)90046-9
10.1126/science.7618104
10.1101/gad.7.7b.1364
10.1073/pnas.89.19.9196
10.1016/0092-8674(87)90576-9
10.1038/337331a0
10.1016/0378-1119(89)90358-2
10.1038/320552a0
10.1016/S0021-9258(18)61060-9 / J. Biol. Chem. / The structure of guanosine-thymidine mismatches in B-DNA at 2.5-Å resolution. (1987)
10.1146/annurev.bi.62.070193.003345
{'key': '2021111418251714000_9.18.2214.18', 'first-page': '6586', 'article-title': 'Sequence-specific DNA primer effects on telomerase polymerization activity.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Sequence-specific DNA primer effects on telomerase polymerization activity. (1993)
10.1101/SQB.1993.058.01.078
10.1016/0092-8674(93)90318-K
10.1101/gad.8.16.1984
10.1002/j.1460-2075.1994.tb06620.x / EMBO J. / Telomerase activity in germline and embryonic cells of Xenopus. (1994)
10.1038/372432a0
10.1073/pnas.87.16.6019
10.1073/pnas.90.4.1493
10.1016/0092-8674(91)90186-3
10.1111/j.1550-7408.1995.tb01537.x / J. Euk. Microbiol. / Circular rDNA replicons persist in Tetrahymena thermophila transformants synthesizing GGGGTC telomeric repeats. (1995)
10.1038/352213a0
10.1126/science.1689074
10.1093/nar/22.6.893
10.1093/nar/19.24.6951
10.1038/239197a0
10.1073/pnas.86.21.8487
10.1016/0092-8674(91)90077-C
10.1016/S0021-9258(19)50101-6 / J. Biol. Chem. / Comparison of HIV-1 and avian myelobastosis virus reverse transcriptase fidelity on RNA and DNA templates. (1992)
10.1038/344126a0
10.1146/annurev.ge.23.120189.003051

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:39 p.m.)
Indexed	1 day, 23 hours ago (Aug. 30, 2025, 1:18 p.m.)
Issued	29 years, 11 months ago (Sept. 15, 1995)
Published	29 years, 11 months ago (Sept. 15, 1995)
Published Online	29 years, 11 months ago (Sept. 15, 1995)
Published Print	29 years, 11 months ago (Sept. 15, 1995)

Funders 0

None

BibTeX

@article{Gilley_1995, title={Altering specific telomerase RNA template residues affects active site function.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.18.2214}, DOI={10.1101/gad.9.18.2214}, number={18}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Gilley, D and Lee, M S and Blackburn, E H}, year={1995}, month=sep, pages={2214–2226} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        30
      ]
    ],
    "date-time": "2025-08-30T17:18:54Z",
    "timestamp": 1756574334410
  },
  "reference-count": 37,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "18",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        9,
        15
      ]
    ]
  },
  "abstract": "<jats:p>The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying a template sequence in the telomerase RNA. We studied the functional roles of specific residues in the Tetrahymena telomerase RNA template region. Unexpectedly, mutation of certain templating residues caused dramatic effects on specific aspects of the enzyme reaction, including loss of enzymatic fidelity and premature product dissociation. None of these fundamental changes in enzymatic action are explainable by altered base-pairing between the telomerase RNA and DNA substrate. These influences of specific template bases of the telomerase RNA on enzymatic properties of telomerase provide evidence for critical roles of these RNA residues in two active site functions--fidelity and DNA substrate/enzyme interaction.</jats:p>",
  "DOI": "10.1101/gad.9.18.2214",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "2214-2226",
  "source": "Crossref",
  "is-referenced-by-count": 74,
  "title": "Altering specific telomerase RNA template residues affects active site function.",
  "prefix": "10.1101",
  "volume": "9",
  "author": [
    {
      "given": "D",
      "family": "Gilley",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "M S",
      "family": "Lee",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "E H",
      "family": "Blackburn",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1995,
        9,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111418251714000_9.18.2214.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.5.563"
    },
    {
      "key": "2021111418251714000_9.18.2214.2",
      "unstructured": "Bebenek, K. and T.A. Kunkel. 1993. The fidelity of retroviral reverse transcriptases. In Reverse transcriptase. (ed. A.M. Skalka and S.P. Goff), pp. 85\u2013103. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York."
    },
    {
      "key": "2021111418251714000_9.18.2214.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8469987"
    },
    {
      "key": "2021111418251714000_9.18.2214.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.5.1758"
    },
    {
      "key": "2021111418251714000_9.18.2214.5",
      "doi-asserted-by": "crossref",
      "first-page": "5721",
      "DOI": "10.1002/j.1460-2075.1994.tb06910.x",
      "article-title": "Architecture of telomerase RNA.",
      "volume": "13",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418251714000_9.18.2214.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8128220"
    },
    {
      "key": "2021111418251714000_9.18.2214.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.61.070192.000553"
    },
    {
      "key": "2021111418251714000_9.18.2214.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90046-9"
    },
    {
      "key": "2021111418251714000_9.18.2214.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7618104"
    },
    {
      "key": "2021111418251714000_9.18.2214.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.7b.1364"
    },
    {
      "key": "2021111418251714000_9.18.2214.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.19.9196"
    },
    {
      "key": "2021111418251714000_9.18.2214.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90576-9"
    },
    {
      "key": "2021111418251714000_9.18.2214.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/337331a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(89)90358-2"
    },
    {
      "key": "2021111418251714000_9.18.2214.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/320552a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.16",
      "doi-asserted-by": "crossref",
      "first-page": "9962",
      "DOI": "10.1016/S0021-9258(18)61060-9",
      "article-title": "The structure of guanosine-thymidine mismatches in B-DNA at 2.5-\u00c5 resolution.",
      "volume": "262",
      "year": "1987",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418251714000_9.18.2214.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.62.070193.003345"
    },
    {
      "key": "2021111418251714000_9.18.2214.18",
      "first-page": "6586",
      "article-title": "Sequence-specific DNA primer effects on telomerase polymerization activity.",
      "volume": "13",
      "year": "1993",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418251714000_9.18.2214.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/SQB.1993.058.01.078"
    },
    {
      "key": "2021111418251714000_9.18.2214.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90318-K"
    },
    {
      "key": "2021111418251714000_9.18.2214.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.16.1984"
    },
    {
      "key": "2021111418251714000_9.18.2214.22",
      "doi-asserted-by": "crossref",
      "first-page": "3211",
      "DOI": "10.1002/j.1460-2075.1994.tb06620.x",
      "article-title": "Telomerase activity in germline and embryonic cells of Xenopus.",
      "volume": "13",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418251714000_9.18.2214.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/372432a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.16.6019"
    },
    {
      "key": "2021111418251714000_9.18.2214.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.4.1493"
    },
    {
      "key": "2021111418251714000_9.18.2214.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90186-3"
    },
    {
      "key": "2021111418251714000_9.18.2214.27",
      "doi-asserted-by": "crossref",
      "first-page": "32",
      "DOI": "10.1111/j.1550-7408.1995.tb01537.x",
      "article-title": "Circular rDNA replicons persist in Tetrahymena thermophila transformants synthesizing GGGGTC telomeric repeats.",
      "volume": "42",
      "year": "1995",
      "journal-title": "J. Euk. Microbiol."
    },
    {
      "key": "2021111418251714000_9.18.2214.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/352213a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1689074"
    },
    {
      "key": "2021111418251714000_9.18.2214.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/22.6.893"
    },
    {
      "key": "2021111418251714000_9.18.2214.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.24.6951"
    },
    {
      "key": "2021111418251714000_9.18.2214.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/239197a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.21.8487"
    },
    {
      "key": "2021111418251714000_9.18.2214.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90077-C"
    },
    {
      "key": "2021111418251714000_9.18.2214.35",
      "doi-asserted-by": "crossref",
      "first-page": "10888",
      "DOI": "10.1016/S0021-9258(19)50101-6",
      "article-title": "Comparison of HIV-1 and avian myelobastosis virus reverse transcriptase fidelity on RNA and DNA templates.",
      "volume": "267",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418251714000_9.18.2214.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/344126a0"
    },
    {
      "key": "2021111418251714000_9.18.2214.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ge.23.120189.003051"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.9.18.2214",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T02:39:36Z",
    "timestamp": 1636943976000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.9.18.2214"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        9,
        15
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "18",
    "published-print": {
      "date-parts": [
        [
          1995,
          9,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.9.18.2214"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.9.18.2214",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1995,
        9,
        15
      ]
    ]
  }
}