Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes.
10.1101/gad.9.16.1953
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have used differential display PCR to search for mRNAs induced by delta Raf-1:ER, an estradiol-dependent form of Raf-1 kinase. Through this approach the gene encoding heparin-binding epidermal growth factor (HB-EGF) was identified as an immediate-early transcriptional target of oncogenic Raf kinases. Activation of delta Raf-1:ER and a conditional oncogenic form of B-Raf, delta B-RAF:ER, resulted in rapid and sustained induction of HB-EGF mRNA expression and secretion of mature HB-EGF from cells. Neutralizing anti-HB-EGF antisera prevented the delayed activation of the c-Jun amino-terminal kinases that is observed in cells transformed by delta Raf-1:ER. These results demonstrate that distinct signaling pathways can cross talk via the secretion of polypeptide growth factors. Furthermore, cells transformed by oncogenic Ras, which also induced HB-EGF expression, demonstrated a marked increase in sensitivity to the cytotoxic action of diphtheria toxin, for which the membrane anchored HB-EGF precursor acts as a cell-surface receptor.

Bibliography

McCarthy, S. A., Samuels, M. L., Pritchard, C. A., Abraham, J. A., & McMahon, M. (1995). Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes. Genes & Development, 9(16), 1953–1964.

Authors 5
  1. S A McCarthy (first)
  2. M L Samuels (additional)
  3. C A Pritchard (additional)
  4. J A Abraham (additional)
  5. M McMahon (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:39 p.m.)
Indexed 3 months ago (May 25, 2025, 9:42 a.m.)
Issued 30 years ago (Aug. 15, 1995)
Published 30 years ago (Aug. 15, 1995)
Published Online 30 years ago (Aug. 15, 1995)
Published Print 30 years ago (Aug. 15, 1995)
Funders 0

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@article{McCarthy_1995, title={Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.16.1953}, DOI={10.1101/gad.9.16.1953}, number={16}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={McCarthy, S A and Samuels, M L and Pritchard, C A and Abraham, J A and McMahon, M}, year={1995}, month=aug, pages={1953–1964} }