DOI: 10.1101/gad.9.13.1572. p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.

p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.

10.1101/gad.9.13.1572

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Fission yeast cold-sensitive (cs) dis1 mutants are defective in sister chromatid separation. The dis1+ gene was isolated by chromosome walking. The null mutant showed the same phenotype as that of cs mutants. The dis1+ gene product was identified as a novel 93-kD protein, and its localization was determined by use of anti-dis1 antibodies and green fluorescent protein (GFP) tagged to the carboxyl end of p93dis1. The tagged p93dis1 in living cells localizes along cytoplasmic microtubule arrays in interphase and the elongating anaphase spindle in mitosis, but association with the short metaphase spindle microtubules is strikingly reduced. In the spindle, the tagged p93dis1 is enriched at the spindle pole bodies (SPBs). Time-lapse video images of single cells support the localization shift of p93dis1 to the SPBs in metaphase and spindle microtubules in anaphase. The carboxy-terminal fragment, which is essential for Dis1 function, accumulates around the mitotic SPB. We propose that these localization shifts of p93dis1 in mitosis facilitates sister chromatid separation by affecting SPB and anaphase spindle function.

Bibliography

Nabeshima, K., Kurooka, H., Takeuchi, M., Kinoshita, K., Nakaseko, Y., & Yanagida, M. (1995). p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites. Genes & Development, 9(13), 1572â1585.

Authors 6

K Nabeshima (first)
H Kurooka (additional)
M Takeuchi (additional)
K Kinoshita (additional)
Y Nakaseko (additional)
M Yanagida (additional)

References 74 Referenced 125

10.1016/S0021-9258(18)92896-6 / J. Biol. Chem. / Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U). (1991)
10.1038/347680a0
10.1016/0092-8674(90)90286-N
10.1038/290140a0
10.1016/0092-8674(90)90022-7
10.1083/jcb.50.2.416
10.1016/0076-6879(91)01013-R
10.1126/science.8303295
10.1016/0092-8674(89)90789-7
10.1083/jcb.120.1.141
10.1016/0092-8674(89)90337-1
10.1016/0378-1119(89)90088-7
10.1083/jcb.121.5.961
10.1083/jcb.119.5.1271
10.1242/jcs.89.3.343 / J. Cell Sci. / The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. (1988)
10.1038/356074a0
10.1083/jcb.129.4.1033
{'key': '2021111418233685000_9.13.1572.18', 'first-page': '517', 'article-title': 'Visualization of the structural polarity of microtubules.', 'volume': '286', 'year': '1980', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. / Visualization of the structural polarity of microtubules. (1980)
10.1083/jcb.106.4.1171
10.1016/0092-8674(84)90013-8
10.1016/0092-8674(93)90364-V
10.1242/jcs.99.4.693 / J. Cell Sci. / The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers. (1991)
10.1016/0092-8674(81)90014-3
10.1128/jb.153.1.163-168.1983 / J. Bacteriol. / Transformation of intact yeast cells treated with alkali cations. (1983)
10.1002/j.1460-2075.1992.tb05489.x / EMBO J. / Microtubule bundling by tau proteins in vivo: Analysis of functional domains. (1992)
10.1016/0092-8674(95)90338-0
10.1016/0092-8674(90)90173-C
10.1101/SQB.1991.056.01.071
{'key': '2021111418233685000_9.13.1572.29', 'first-page': '5839', 'article-title': 'The fission yeast dis3\n + gene encodes a 110-kDa essential protein implicated in mitotic control.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The fission yeast dis3 + gene encodes a 110-kDa essential protein implicated in mitotic control. (1991)
10.1101/gad.7.6.1059
10.1016/0092-8674(81)90015-5
Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1083/jcb.117.5.1055
{'key': '2021111418233685000_9.13.1572.34', 'first-page': '4424', 'article-title': 'Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe. (1987)
{'key': '2021111418233685000_9.13.1572.35', 'first-page': '10857', 'article-title': 'nmt1 of fission yeast.', 'volume': '265', 'year': '1990', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / nmt1 of fission yeast. (1990)
10.1016/0092-8674(93)90080-A
10.1242/jcs.9.2.475 / J. Cell Sci. / Mitosis in the fission yeast Schizosaccharomyces pombe: A comparative study with light and electron microscopy. (1971)
10.1016/0092-8674(93)90377-3
10.1016/0092-8674(90)90351-E
10.1083/jcb.120.4.959
10.1016/S0091-679X(08)61752-5
10.1038/312237a0
10.1038/344503a0
10.1016/0962-8924(92)90125-7
10.1002/j.1460-2075.1988.tb02964.x / EMBO J. / Cold-sensitive and caffeine supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis. (1988)
10.1016/0092-8674(89)90338-3
10.1083/jcb.125.4.853
10.1101/gad.6.8.1414
10.1242/jcs.106.1.87 / J. Cell Sci. / Mutations in the Drosophila melanogaster gene three rows permit aspects of mitosis to continue in the absence of chromatid segregation. (1993)
Pichová, A., Kohlwein, S.D. and Yamamoto. M. 1995. New arrays of cytoplasmic microtubules in the fission yeast Schizosaccharomyces pombe. Protoplasma (in press). (10.1007/BF01280377)
10.1083/jcb.111.5.1913
10.1073/pnas.74.12.5463
10.1126/science.7939665
10.1091/mbc.4.3.303
10.1083/jcb.119.5.1023
10.1016/0092-8674(86)90390-9
10.1007/BF00362281 / Chromosoma / The spindle pole body of yeast. (1994)
10.1016/S0092-8674(88)90977-4
10.1073/pnas.89.19.8908
10.1016/0960-9822(93)90140-J
10.1016/0022-2836(86)90385-2
10.1091/mbc.4.3.247
10.1242/jcs.80.1.253 / J. Cell Sci. / Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy. (1986)
10.1073/pnas.76.9.4350
10.1016/0092-8674(95)90336-4
10.1016/0092-8674(91)90511-V
10.1242/jcs.101.1.79 / J. Cell Sci. / Inhibition of mitosis by okadaic acid: Possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase. (1992)
10.1083/jcb.118.5.1097
10.1083/jcb.107.4.1437
{'key': '2021111418233685000_9.13.1572.70', 'first-page': '448', 'article-title': 'Expression and characterization of the human c-myc DNA-binding protein.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / Expression and characterization of the human c-myc DNA-binding protein. (1985)
10.1242/jcs.93.3.491 / J. Cell Sci. / Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. ((1989))
10.1002/j.1460-2075.1994.tb06865.x / EMBO J. / Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. (1994)
Yanagida, M., O. Niwa, Y. Chikashige, T. Matsumoto, S. Murakami, H. Kurooka, T. Horio, and M. Shimanuki. 1991. Genome analysis of Schizosaccharomyces pombe. In Control of cell growth and division. (ed. A. Ishihama and H. Yoshikawa), pp. 255–262. Japan Sci. Soc. Press/Springer-Verlag, Tokyo, Japan.
10.1016/0378-1119(85)90120-9

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:34 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 5, 2025, 8:20 a.m.)
Issued	30 years, 1 month ago (July 1, 1995)
Published	30 years, 1 month ago (July 1, 1995)
Published Online	30 years, 1 month ago (July 1, 1995)
Published Print	30 years, 1 month ago (July 1, 1995)

Funders 0

None

BibTeX

@article{Nabeshima_1995, title={p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.13.1572}, DOI={10.1101/gad.9.13.1572}, number={13}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Nabeshima, K and Kurooka, H and Takeuchi, M and Kinoshita, K and Nakaseko, Y and Yanagida, M}, year={1995}, month=jul, pages={1572–1585} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:20:54Z",
    "timestamp": 1754396454576
  },
  "reference-count": 74,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "13",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        7,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Fission yeast cold-sensitive (cs) dis1 mutants are defective in sister chromatid separation. The dis1+ gene was isolated by chromosome walking. The null mutant showed the same phenotype as that of cs mutants. The dis1+ gene product was identified as a novel 93-kD protein, and its localization was determined by use of anti-dis1 antibodies and green fluorescent protein (GFP) tagged to the carboxyl end of p93dis1. The tagged p93dis1 in living cells localizes along cytoplasmic microtubule arrays in interphase and the elongating anaphase spindle in mitosis, but association with the short metaphase spindle microtubules is strikingly reduced. In the spindle, the tagged p93dis1 is enriched at the spindle pole bodies (SPBs). Time-lapse video images of single cells support the localization shift of p93dis1 to the SPBs in metaphase and spindle microtubules in anaphase. The carboxy-terminal fragment, which is essential for Dis1 function, accumulates around the mitotic SPB. We propose that these localization shifts of p93dis1 in mitosis facilitates sister chromatid separation by affecting SPB and anaphase spindle function.</jats:p>",
  "DOI": "10.1101/gad.9.13.1572",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "1572-1585",
  "source": "Crossref",
  "is-referenced-by-count": 125,
  "title": "p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.",
  "prefix": "10.1101",
  "volume": "9",
  "author": [
    {
      "given": "K",
      "family": "Nabeshima",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "H",
      "family": "Kurooka",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M",
      "family": "Takeuchi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "K",
      "family": "Kinoshita",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Y",
      "family": "Nakaseko",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M",
      "family": "Yanagida",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1995,
        7,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111418233685000_9.13.1572.1",
      "doi-asserted-by": "crossref",
      "first-page": "9841",
      "DOI": "10.1016/S0021-9258(18)92896-6",
      "article-title": "Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U).",
      "volume": "266",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418233685000_9.13.1572.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/347680a0"
    },
    {
      "key": "2021111418233685000_9.13.1572.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90286-N"
    },
    {
      "key": "2021111418233685000_9.13.1572.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/290140a0"
    },
    {
      "key": "2021111418233685000_9.13.1572.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90022-7"
    },
    {
      "key": "2021111418233685000_9.13.1572.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.50.2.416"
    },
    {
      "key": "2021111418233685000_9.13.1572.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(91)01013-R"
    },
    {
      "key": "2021111418233685000_9.13.1572.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8303295"
    },
    {
      "key": "2021111418233685000_9.13.1572.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90789-7"
    },
    {
      "key": "2021111418233685000_9.13.1572.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.120.1.141"
    },
    {
      "key": "2021111418233685000_9.13.1572.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90337-1"
    },
    {
      "key": "2021111418233685000_9.13.1572.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(89)90088-7"
    },
    {
      "key": "2021111418233685000_9.13.1572.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.121.5.961"
    },
    {
      "key": "2021111418233685000_9.13.1572.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.119.5.1271"
    },
    {
      "key": "2021111418233685000_9.13.1572.15",
      "doi-asserted-by": "crossref",
      "first-page": "343",
      "DOI": "10.1242/jcs.89.3.343",
      "article-title": "The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.",
      "volume": "89",
      "year": "1988",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/356074a0"
    },
    {
      "key": "2021111418233685000_9.13.1572.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.129.4.1033"
    },
    {
      "key": "2021111418233685000_9.13.1572.18",
      "first-page": "517",
      "article-title": "Visualization of the structural polarity of microtubules.",
      "volume": "286",
      "year": "1980",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2021111418233685000_9.13.1572.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.106.4.1171"
    },
    {
      "key": "2021111418233685000_9.13.1572.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90013-8"
    },
    {
      "key": "2021111418233685000_9.13.1572.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90364-V"
    },
    {
      "key": "2021111418233685000_9.13.1572.22",
      "doi-asserted-by": "crossref",
      "first-page": "693",
      "DOI": "10.1242/jcs.99.4.693",
      "article-title": "The fission yeast \u03b3-tubulin is essential for mitosis and is localized at microtubule organizing centers.",
      "volume": "99",
      "year": "1991",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90014-3"
    },
    {
      "key": "2021111418233685000_9.13.1572.24",
      "doi-asserted-by": "crossref",
      "first-page": "163",
      "DOI": "10.1128/jb.153.1.163-168.1983",
      "article-title": "Transformation of intact yeast cells treated with alkali cations.",
      "volume": "153",
      "year": "1983",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "2021111418233685000_9.13.1572.25",
      "doi-asserted-by": "crossref",
      "first-page": "3953",
      "DOI": "10.1002/j.1460-2075.1992.tb05489.x",
      "article-title": "Microtubule bundling by tau proteins in vivo: Analysis of functional domains.",
      "volume": "11",
      "year": "1992",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418233685000_9.13.1572.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90338-0"
    },
    {
      "key": "2021111418233685000_9.13.1572.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90173-C"
    },
    {
      "key": "2021111418233685000_9.13.1572.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/SQB.1991.056.01.071"
    },
    {
      "key": "2021111418233685000_9.13.1572.29",
      "first-page": "5839",
      "article-title": "The fission yeast dis3\n               + gene encodes a 110-kDa essential protein implicated in mitotic control.",
      "volume": "11",
      "year": "1991",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418233685000_9.13.1572.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.6.1059"
    },
    {
      "key": "2021111418233685000_9.13.1572.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90015-5"
    },
    {
      "key": "2021111418233685000_9.13.1572.32",
      "unstructured": "Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
    },
    {
      "key": "2021111418233685000_9.13.1572.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.117.5.1055"
    },
    {
      "key": "2021111418233685000_9.13.1572.34",
      "first-page": "4424",
      "article-title": "Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe.",
      "volume": "7",
      "year": "1987",
      "journal-title": "Mol. Cell Biol."
    },
    {
      "key": "2021111418233685000_9.13.1572.35",
      "first-page": "10857",
      "article-title": "nmt1 of fission yeast.",
      "volume": "265",
      "year": "1990",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418233685000_9.13.1572.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90080-A"
    },
    {
      "key": "2021111418233685000_9.13.1572.37",
      "doi-asserted-by": "crossref",
      "first-page": "475",
      "DOI": "10.1242/jcs.9.2.475",
      "article-title": "Mitosis in the fission yeast Schizosaccharomyces pombe: A comparative study with light and electron microscopy.",
      "volume": "9",
      "year": "1971",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90377-3"
    },
    {
      "key": "2021111418233685000_9.13.1572.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90351-E"
    },
    {
      "key": "2021111418233685000_9.13.1572.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.120.4.959"
    },
    {
      "key": "2021111418233685000_9.13.1572.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(08)61752-5"
    },
    {
      "key": "2021111418233685000_9.13.1572.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/312237a0"
    },
    {
      "key": "2021111418233685000_9.13.1572.43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/344503a0"
    },
    {
      "key": "2021111418233685000_9.13.1572.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0962-8924(92)90125-7"
    },
    {
      "key": "2021111418233685000_9.13.1572.45",
      "doi-asserted-by": "crossref",
      "first-page": "1465",
      "DOI": "10.1002/j.1460-2075.1988.tb02964.x",
      "article-title": "Cold-sensitive and caffeine supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.",
      "volume": "7",
      "year": "1988",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418233685000_9.13.1572.46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90338-3"
    },
    {
      "key": "2021111418233685000_9.13.1572.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.125.4.853"
    },
    {
      "key": "2021111418233685000_9.13.1572.48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.8.1414"
    },
    {
      "key": "2021111418233685000_9.13.1572.49",
      "doi-asserted-by": "crossref",
      "first-page": "87",
      "DOI": "10.1242/jcs.106.1.87",
      "article-title": "Mutations in the Drosophila melanogaster gene three rows permit aspects of mitosis to continue in the absence of chromatid segregation.",
      "volume": "106",
      "year": "1993",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.50",
      "doi-asserted-by": "crossref",
      "unstructured": "Pichov\u00e1, A., Kohlwein, S.D. and Yamamoto. M. 1995. New arrays of cytoplasmic microtubules in the fission yeast Schizosaccharomyces pombe. Protoplasma (in press).",
      "DOI": "10.1007/BF01280377"
    },
    {
      "key": "2021111418233685000_9.13.1572.51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.111.5.1913"
    },
    {
      "key": "2021111418233685000_9.13.1572.52",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.74.12.5463"
    },
    {
      "key": "2021111418233685000_9.13.1572.53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7939665"
    },
    {
      "key": "2021111418233685000_9.13.1572.54",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.4.3.303"
    },
    {
      "key": "2021111418233685000_9.13.1572.55",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.119.5.1023"
    },
    {
      "key": "2021111418233685000_9.13.1572.56",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90390-9"
    },
    {
      "key": "2021111418233685000_9.13.1572.57",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1007/BF00362281",
      "article-title": "The spindle pole body of yeast.",
      "volume": "103",
      "year": "1994",
      "journal-title": "Chromosoma"
    },
    {
      "key": "2021111418233685000_9.13.1572.58",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(88)90977-4"
    },
    {
      "key": "2021111418233685000_9.13.1572.59",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.19.8908"
    },
    {
      "key": "2021111418233685000_9.13.1572.60",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0960-9822(93)90140-J"
    },
    {
      "key": "2021111418233685000_9.13.1572.61",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(86)90385-2"
    },
    {
      "key": "2021111418233685000_9.13.1572.62",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.4.3.247"
    },
    {
      "key": "2021111418233685000_9.13.1572.63",
      "doi-asserted-by": "crossref",
      "first-page": "253",
      "DOI": "10.1242/jcs.80.1.253",
      "article-title": "Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy.",
      "volume": "80",
      "year": "1986",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.64",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.9.4350"
    },
    {
      "key": "2021111418233685000_9.13.1572.65",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90336-4"
    },
    {
      "key": "2021111418233685000_9.13.1572.66",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90511-V"
    },
    {
      "key": "2021111418233685000_9.13.1572.67",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1242/jcs.101.1.79",
      "article-title": "Inhibition of mitosis by okadaic acid: Possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase.",
      "volume": "101",
      "year": "1992",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.68",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.118.5.1097"
    },
    {
      "key": "2021111418233685000_9.13.1572.69",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.107.4.1437"
    },
    {
      "key": "2021111418233685000_9.13.1572.70",
      "first-page": "448",
      "article-title": "Expression and characterization of the human c-myc DNA-binding protein.",
      "volume": "5",
      "year": "1985",
      "journal-title": "Mol. Cell Biol."
    },
    {
      "key": "2021111418233685000_9.13.1572.71",
      "doi-asserted-by": "crossref",
      "first-page": "491",
      "DOI": "10.1242/jcs.93.3.491",
      "article-title": "Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies.",
      "volume": "93",
      "year": "(1989)",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111418233685000_9.13.1572.72",
      "doi-asserted-by": "crossref",
      "first-page": "5310",
      "DOI": "10.1002/j.1460-2075.1994.tb06865.x",
      "article-title": "Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation.",
      "volume": "13",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418233685000_9.13.1572.73",
      "unstructured": "Yanagida, M., O. Niwa, Y. Chikashige, T. Matsumoto, S. Murakami, H. Kurooka, T. Horio, and M. Shimanuki. 1991. Genome analysis of Schizosaccharomyces pombe. In Control of cell growth and division. (ed. A. Ishihama and H. Yoshikawa), pp. 255\u2013262. Japan Sci. Soc. Press/Springer-Verlag, Tokyo, Japan."
    },
    {
      "key": "2021111418233685000_9.13.1572.74",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(85)90120-9"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.9.13.1572",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T02:34:51Z",
    "timestamp": 1636943691000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.9.13.1572"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        7,
        1
      ]
    ]
  },
  "references-count": 74,
  "journal-issue": {
    "issue": "13",
    "published-print": {
      "date-parts": [
        [
          1995,
          7,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.9.13.1572"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.9.13.1572",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1995,
        7,
        1
      ]
    ]
  }
}