Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.
10.1101/gad.9.11.1400
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Transient transfection of the U1 snRNP 70K protein into COS cells induced nuclear reorganization and redistribution of the splicing factor SC-35, whereas hnRNP proteins were not affected. Correspondingly, splicing and nucleocytoplasmic transport of a coexpressed mRNA substrate was reduced by overexpression of U1-70K. The carboxy-terminal portion of U1-70K-encompassing repeats of Arg/Ser, Arg/Glu, and Arg/Asp localizes to the nucleus independently of U1 RNA and was responsible for these inhibitory effects. This region of U1-70K contains amino acid residues similar to those found in splicing factors SC-35, U2AF, su(wa), and in other SR proteins suggesting that U1-70K protein may serve as a focus of assembly for functional components of the splicing/transport machinery. These findings are compatible with models that propose that direct interaction between U1-70K and SR proteins play a regulatory role in early events of spliceosome assembly.

Bibliography

Romac, J. M., & Keene, J. D. (1995). Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA. Genes & Development, 9(11), 1400–1410.

Authors 2
  1. J M Romac (first)
  2. J D Keene (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:34 p.m.)
Indexed 3 weeks, 2 days ago (Aug. 5, 2025, 8:53 a.m.)
Issued 30 years, 2 months ago (June 1, 1995)
Published 30 years, 2 months ago (June 1, 1995)
Published Online 30 years, 2 months ago (June 1, 1995)
Published Print 30 years, 2 months ago (June 1, 1995)
Funders 0

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@article{Romac_1995, title={Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.11.1400}, DOI={10.1101/gad.9.11.1400}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Romac, J M and Keene, J D}, year={1995}, month=jun, pages={1400–1410} }