Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator.
10.1101/gad.9.11.1377
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The immunoglobulin light-chain gene enhancers E kappa 3', E lambda 2-4, and E lambda 3-1 contain a conserved cell type-specific composite element essential for their activities. This element binds a B cell-specific heterodimeric protein complex that consists of the Ets family member PU.1 and a second factor (NF-EM5), whose participation in the formation of the complex is dependent on the presence of DNA-bound PU.1. In this report we describe the cloning and characterization of Pip (PU.1 interaction partner), a lymphoid-specific protein that is most likely NF-EM5. As expected, the Pip protein binds the composite element only in the presence of PU.1; furthermore, the formation of this ternary complex is critically dependent on phosphorylation of PU.1 at serine-148. The Pip gene is expressed specifically in lymphoid tissues in both B- and T-cell lines. When coexpressed in NIH-3T3 cells, Pip and PU.1 function as mutually dependent transcription activators of the composite element. The amino-terminal DNA-binding domain of Pip exhibits a high degree of homology to the DNA-binding domains of members of the interferon regulatory factor (IRF) family, which includes IRF-1, IRF-2, ICSBP, and ISGF3 gamma.

Bibliography

Eisenbeis, C. F., Singh, H., & Storb, U. (1995). Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator. Genes & Development, 9(11), 1377–1387.

Authors 3
  1. C F Eisenbeis (first)
  2. H Singh (additional)
  3. U Storb (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 1 year, 6 months ago (Feb. 14, 2024, 4:28 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:33 p.m.)
Issued 30 years, 2 months ago (June 1, 1995)
Published 30 years, 2 months ago (June 1, 1995)
Published Online 30 years, 2 months ago (June 1, 1995)
Published Print 30 years, 2 months ago (June 1, 1995)
Funders 0

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@article{Eisenbeis_1995, title={Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator.}, volume={9}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.9.11.1377}, DOI={10.1101/gad.9.11.1377}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Eisenbeis, C F and Singh, H and Storb, U}, year={1995}, month=jun, pages={1377–1387} }