DOI: 10.1101/gad.891401. Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin

Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin

10.1101/gad.891401

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

In skin, multipotent stem cells generate the keratinocytes of the epidermis, sebaceous gland, and hair follicles. In this paper, we show that Tcf3 and Lef1 control these differentiation lineages. In contrast to Lef1, which requires Wnt signaling and stabilized β-catenin to express the hair-specific keratin genes and control hair differentiation, Tcf3 can act independently of its β-catenin interacting domain to suppress features of epidermal terminal differentiation, in which Tcf3 is normally shut off, and promote features of the follicle outer root sheath (ORS) and multipotent stem cells (bulge), the compartments which naturally express Tcf3. These aspects of Tcf3's action are dependent on its DNA binding and Groucho repressor-binding domains. In the absence of its β-catenin interacting domain, Lef1's behavior (ΔNLef1) seems to be markedly distinct from that of ΔNTcf3. ΔNLef1 does not suppress epidermal differentiation and promote ORS/bulge differentiation, but rather suppresses hair differentiation and gives rise to sebocyte differentiation. Taken together, these findings provide powerful evidence that the status of Tcf3/Lef complexes has a key role in controlling cell fate lineages in multipotent skin stem cells.

Bibliography

Merrill, B. J., Gat, U., DasGupta, R., & Fuchs, E. (2001). Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin. Genes & Development, 15(13), 1688â1705.

Authors 4

Bradley J. Merrill (first)
Uri Gat (additional)
Ramanuj DasGupta (additional)
Elaine Fuchs (additional)

References 41 Referenced 477

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{'key': '2021111507404469000_15.13.1688.8', 'first-page': '47', 'article-title': 'Beauty is skin deep: The fascinating biology of the epidermis and its appendages.', 'volume': '94', 'author': 'Fuchs', 'year': '1997', 'journal-title': 'Harvey Lect.'} / Harvey Lect. / Beauty is skin deep: The fascinating biology of the epidermis and its appendages. by Fuchs (1997)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:43 a.m.)
Indexed	1 month, 4 weeks ago (July 8, 2025, 3:05 a.m.)
Issued	24 years, 2 months ago (July 1, 2001)
Published	24 years, 2 months ago (July 1, 2001)
Published Online	24 years, 2 months ago (July 1, 2001)
Published Print	24 years, 2 months ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Merrill_2001, title={Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.891401}, DOI={10.1101/gad.891401}, number={13}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Merrill, Bradley J. and Gat, Uri and DasGupta, Ramanuj and Fuchs, Elaine}, year={2001}, month=jul, pages={1688–1705} }

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