DOI: 10.1101/gad.888601. TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm

TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm

10.1101/gad.888601

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Mammals express four highly conserved TEAD/TEF transcription factors that bind the same DNA sequence, but serve different functions during development. TEAD-2/TEF-4 protein purified from mouse cells was associated predominantly with a novel TEAD-binding domain at the amino terminus of YAP65, a powerful transcriptional coactivator. YAP65 interacted specifically with the carboxyl terminus of all four TEAD proteins. Both this interaction and sequence-specific DNA binding by TEAD were required for transcriptional activation in mouse cells. Expression of YAP in lymphocytic cells that normally do not support TEAD-dependent transcription (e.g., MPC11) resulted in up to 300-fold induction of TEAD activity. Conversely, TEAD overexpression squelched YAP activity. Therefore, the carboxy-terminal acidic activation domain in YAP is the transcriptional activation domain for TEAD transcription factors. However, whereas TEAD was concentrated in the nucleus, excess YAP65 accumulated in the cytoplasm as a complex with the cytoplasmic localization protein, 14-3-3. Because TEAD-dependent transcription was limited by YAP65, and YAP65 also binds Src/Yes protein tyrosine kinases, we propose that YAP65 regulates TEAD-dependent transcription in response to mitogenic signals.

Bibliography

Vassilev, A., Kaneko, K. J., Shu, H., Zhao, Y., & DePamphilis, M. L. (2001). TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm. Genes & Development, 15(10), 1229â1241.

Authors 5

Alex Vassilev (first)
Kotaro J. Kaneko (additional)
Hongjun Shu (additional)
Yingming Zhao (additional)
Melvin L. DePamphilis (additional)

References 37 Referenced 626

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{'key': '2021111507392243000_15.10.1229.30', 'first-page': '2145', 'article-title': 'Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product.', 'volume': '9', 'author': 'Sudol', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene / Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product. by Sudol (1994)
10.1074/jbc.270.24.14733
10.1074/jbc.M001970200
10.1242/dev.126.21.4807 / Development / TONDU (TDU), a novel human protein related to the product of vestigial (vg) gene of Drosophila melanogaster interacts with vertebrate TEF factors and substitutes for Vg function in wing formation. by Vaudin (1999)
10.1095/biolreprod62.4.969
10.1016/0092-8674(91)90088-G
10.1093/emboj/18.9.2551
10.1074/jbc.271.7.3727

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:40 a.m.)
Indexed	1 day, 15 hours ago (Sept. 4, 2025, 9:22 a.m.)
Issued	24 years, 3 months ago (May 15, 2001)
Published	24 years, 3 months ago (May 15, 2001)
Published Online	24 years, 3 months ago (May 15, 2001)
Published Print	24 years, 3 months ago (May 15, 2001)

Funders 0

None

BibTeX

@article{Vassilev_2001, title={TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.888601}, DOI={10.1101/gad.888601}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Vassilev, Alex and Kaneko, Kotaro J. and Shu, Hongjun and Zhao, Yingming and DePamphilis, Melvin L.}, year={2001}, month=may, pages={1229–1241} }

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