DOI: 10.1101/gad.832000. Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase

Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase

10.1101/gad.832000

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Cohesin complex acts in the formation and maintenance of sister chromatid cohesion during and after S phase. Budding yeast Scc1p/Mcd1p, an essential subunit, is cleaved and dissociates from chromosomes in anaphase, leading to sister chromatid separation. Most cohesin in higher eukaryotes, in contrast, is dissociated from chromosomes well before anaphase. The universal role of cohesin during anaphase thus remains to be determined. We report here initial characterization of four putative cohesin subunits, Psm1, Psm3, Rad21, and Psc3, in fission yeast. They are essential for sister chromatid cohesion. Immunoprecipitation demonstrates stable complex formation of Rad21 with Psm1 and Psm3 but not with Psc3. Chromatin immunoprecipitation shows that cohesin subunits are enriched in broad centromere regions and that the level of centromere-associated Rad21 did not change from metaphase to anaphase, very different from budding yeast. In contrast, Rad21 containing similar cleavage sites to those of Scc1p/Mcd1p is cleaved specifically in anaphase. This cleavage is essential, although the amount of cleaved product is very small (<5%). Mis4, another sister chromatid cohesion protein, plays an essential role for loading Rad21 on chromatin. A simple model is presented to explain the specific behavior of fission yeast cohesin and why only a tiny fraction of Rad21 is sufficient to be cleaved for normal anaphase.

Bibliography

Tomonaga, T., Nagao, K., Kawasaki, Y., Furuya, K., Murakami, A., Morishita, J., Yuasa, T., Sutani, T., Kearsey, S. E., Uhlmann, F., Nasmyth, K., & Yanagida, M. (2000). Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase. Genes & Development, 14(21), 2757â2770.

Authors 12

Takeshi Tomonaga (first)
Koji Nagao (additional)
Yosuke Kawasaki (additional)
Kanji Furuya (additional)
Akiko Murakami (additional)
Jun Morishita (additional)
Tatsuro Yuasa (additional)
Takashi Sutani (additional)
Stephen E. Kearsey (additional)
Frank Uhlmann (additional)
Kim Nasmyth (additional)
Mitsuhiro Yanagida (additional)

References 55 Referenced 237

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Dates

Type	When
Created	23 years ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:38 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 7, 2025, 4:49 p.m.)
Issued	24 years, 9 months ago (Nov. 1, 2000)
Published	24 years, 9 months ago (Nov. 1, 2000)
Published Online	24 years, 9 months ago (Nov. 1, 2000)
Published Print	24 years, 9 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Tomonaga_2000, title={Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.832000}, DOI={10.1101/gad.832000}, number={21}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Tomonaga, Takeshi and Nagao, Koji and Kawasaki, Yosuke and Furuya, Kanji and Murakami, Akiko and Morishita, Jun and Yuasa, Tatsuro and Sutani, Takashi and Kearsey, Stephen E. and Uhlmann, Frank and Nasmyth, Kim and Yanagida, Mitsuhiro}, year={2000}, month=nov, pages={2757–2770} }

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