DOI: 10.1101/gad.826100. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

10.1101/gad.826100

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coliRecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein–protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

Bibliography

Petukhova, G., Sung, P., & Klein, H. (2000). Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes & Development, 14(17), 2206â2215.

Authors 3

Galina Petukhova (first)
Patrick Sung (additional)
Hannah Klein (additional)

References 27 Referenced 123

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Dates

Type	When
Created	23 years ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:37 p.m.)
Indexed	3 months, 3 weeks ago (April 30, 2025, 8:33 p.m.)
Issued	24 years, 11 months ago (Sept. 1, 2000)
Published	24 years, 11 months ago (Sept. 1, 2000)
Published Online	24 years, 11 months ago (Sept. 1, 2000)
Published Print	24 years, 11 months ago (Sept. 1, 2000)

Funders 0

None

BibTeX

@article{Petukhova_2000, title={Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.826100}, DOI={10.1101/gad.826100}, number={17}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Petukhova, Galina and Sung, Patrick and Klein, Hannah}, year={2000}, month=sep, pages={2206–2215} }

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